Second Harmonic Imaging Microscopy
Nonlinear System, Second-Harmonic Generation
Herausgegeben: Quincy, Ferdinand M.
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Second harmonic imaging microscopy (SHIM) is based on a nonlinear optical effect known as second harmonic generation (SHG). SHIM has been established as a viable microscope imaging contrast mechanism for visualization of cell and tissue structure and function. A second harmonic microscope obtains contrasts from variations in a specimen s ability to generate second harmonic light from the incident light while a conventional optical microscope obtains its contrast by detecting variations in optical density, path length, or refractive index of the specimen. SHG requires intense laser light passin...
Second harmonic imaging microscopy (SHIM) is based on a nonlinear optical effect known as second harmonic generation (SHG). SHIM has been established as a viable microscope imaging contrast mechanism for visualization of cell and tissue structure and function. A second harmonic microscope obtains contrasts from variations in a specimen s ability to generate second harmonic light from the incident light while a conventional optical microscope obtains its contrast by detecting variations in optical density, path length, or refractive index of the specimen. SHG requires intense laser light passing through a material with a noncentrosymmetric molecular structure. Second-harmonic light emerging from an SHG material is exactly half the wavelength (frequency doubled) of the light entering the material.
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