Shan S. Wong, David M. Jameson

Chemistry of Protein and Nucleic Acid Cross-Linking and Conjugation (eBook, PDF)

• Format: PDF

Produktbeschreibung

An overview of the chemical principles underlying the processes of cross-linking and conjugation, this book offers a thorough list of cross-linking reagents published in the literature since the first edition, covering monofunctional, homobifunctional, heterobifunctional, multifunctional, and zero-length cross-linkers. It reviews of the use of these reagents in studying protein tertiary structures, geometric arrangements of subunits within complex proteins and nucleic acids, near-neighbor analysis, protein-to-protein or ligand-receptor interactions, and conformational changes of biomolecules. Finally, it discusses the application of immunoconjugation for immunoassays, immunotoxins for targeted therapy, microarray technology for analysis of various biomolecules, and solid state chemistry for immobilizations.

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Produktdetails

• Verlag: Taylor & Francis
• Seitenzahl: 622
• Erscheinungstermin: 10. Oktober 2011
• Englisch
• ISBN-13: 9781420007640
• Artikelnr.: 39142433

Autorenporträt

Shan S. Wong, Ph.D., recently retired from the National Institutes of Health, where he served as a scientific review administrator and a program officer. In the latter capacity, he oversaw scientific programs in the area of alternative and complementary medicine. Previously, he served as director of clinical chemistry at Hermann Hospital and Lyndon B. Johnson General Hospital in Houston, Texas, and as a faculty member at the University of Texas Health Science Center at Houston. Before joining the University of Texas, Dr. Wong was a full professor of chemistry at the University of Massachusetts at Lowell. In addition to teaching at the University of Massachusetts at Lowell, he also taught chemistry courses at Denison University, Granville, Ohio, and Ohio State University, Columbus.

Dr. Wong has published extensively in various scientific journals in the area of enzymology and clinical chemistry. He has received numerous honors and awards and has been active in various professional societies.

David M. Jameson, Ph.D., joined the Department of Cell and Molecular Biology at the John A. Burns School of Medicine at the University of Hawaii in 1989, where he is presently a full professor. Before moving to Hawaii he was on the faculty of the Pharmacology Department at the University of Texas Southwestern Medical School in Dallas.

Dr. Jameson received his BS in chemistry from Ohio State University in 1971 and his PhD in biochemistry from the University of Illinois at Urbana-Champaign in 1978. His thesis advisor was Gregorio Weber, who laid the foundations of modern fluorescence spectroscopy. Dr. Jameson carried out postdoctoral research at the Universite Paris-Sud at Orsay, France before returning to the University of Illinois for a postdoctoral period in Gregorio Weber's laboratory. In 1983, he joined the Pharmacology Department at the University of Texas Southwestern Medical Center at Dallas as an assistant profess

Inhaltsangabe

Overview of Protein Conjugation. Review of Protein and Nucleic Acid Chemistry. Reagents Targeted to Specific Functional Groups. How to Design and Choose Cross
Linking Reagents. Homobifunctional Cross
Linking Reagents. Heterobifunctional Cross
Linkers. Multifunctional Cross
Linking Reagents. Monofunctional and Zero
Length Cross
Linking Reagents. General Approaches for Chemical Cross
Linking. Analysis of Cross
Linked Products. Applications of Chemical Cross
Linking to the Study of Biological Macromolecules. Applications of Chemical Conjugation in the Preparation of Immunoconjugates and Immunogens. Application of Chemical Conjugation for the Preparation of Immunotoxins and Other Drug Conjugates for Targeting Therapeutics. Application of Chemical Conjugation to Solid
State Chemistry. Amino Group
Directed Homobifunctional Cross
Linkers. Sulfhydryl Group
Directed Homobifunctional Cross
Linkers. Phenolate
and Imidazolyl
Group
Directed Reagents: Bisdiazonium Precursors. Group Selective Heterobifunctional Cross
Linkers. Photoactivatable Heterobifunctional Cross
Linking Reagents.