Life at the Nanoscale (eBook, ePUB)

Atomic Force Microscopy of Live Cells
Redaktion: Dufrene, Yves

• Format: ePub

Produktbeschreibung

Proceeding from basic fundamentals to applications, this volume provides a comprehensive overview of the use of AFM and related scanning probe microscopies for cell surface analysis. It covers all cell types, from viruses and protoplasts to bacteria and animal cells. It also discusses a range of advanced AFM modalities, including high-resolution imaging, nanoindentation measurements, recognition imaging, and single-molecule and single-cell force spectroscopy. The book covers methodologies for preparing and analyzing cells and membranes of all kinds and highlights recent examples to illustrate the power of AFM techniques in life sciences and nanomedicine.

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Produktdetails

• Verlag: Taylor & Francis
• Seitenzahl: 460
• Erscheinungstermin: 28. März 2019
• Englisch
• ISBN-13: 9780429533808
• Artikelnr.: 56289385

Autorenporträt

Yves Dufrêne received his engineering degree in chemistry and bioindustries (in 1991) and a PhD degree in biophysical chemistry (in 1996) at the Université catholique de Louvain (UCL, Belgium). After a postdoc at the Naval Research Laboratory (Washington DC, USA), he became research associate (2000) of the Belgian National Fund for Scientific Research and lecturer in physical chemistry and nanobiotechnology at UCL. He is currently the head of the Laboratory of Chemistry of Interfaces.

Inhaltsangabe

Observing the Nanoscale Organization of Model Biological Membranes by
Atomic Force Microscopy. High-Resolution Atomic Force Microscopy of Native
Membranes. Microbial Cell Imaging Using Atomic Force Microscopy. Resolving
the High-Resolution Architecture, Assembly and Functional Repertoire of
Bacterial Systems by Atomic Force Microscopy. Understanding Cell Secretion
and Membrane Fusion Processes on the Nanoscale Using the Atomic Force
Microscope. Nanophysiology of Cells, Channels and Nuclear Pores. Topography
and Recognition Imaging of Cells. High-Speed Atomic Force Microscopy for
Dynamic Biological Imaging. Near-Field Scanning Optical Microscopy of
Biological Membranes. Quantifying Cell Adhesion Using Single-Cell Force
Spectroscopy. Probing Cellular Adhesion at the Single-Molecule Level.
Mapping Membrane Proteins on Living Cells Using the Atomic Force
Microscope. Probing Bacterial Adhesion Using Force Spectroscopy. Force
Spectroscopy of Mineral-Microbe Bonds. Single-Molecule Force Spectroscopy
of Microbial Cell Envelope Proteins. Probing the Nanomechanical Properties
of Viruses, Cells and Cellular Structures. Label-Free Monitoring of Cell
Signalling Processes Through AFM-Based Force Measurements. Investigating
Mammalian Cell Nanomechanics with Simultaneous Optical and Atomic Force
Microscopy. The Role of Atomic Force Microscopy in Advancing Diatom
Research into the Nanotechnology Era. Atomic Force Microscopy for Medicine.