Richard W. Burry

Immunocytochemistry (eBook, PDF)

A Practical Guide for Biomedical Research

• Format: PDF

Produktbeschreibung

Immunocytochemistry is a microscopic method that uses antibodies to localize proteins in tissue sections and cell cultures. Dr. Burry's book is the first practical guide to planning, performing, and evaluating immunocytochemical experiments designed for novice immunocytochemists. This book's predominant orientation is academic, focusing only on the most productive methods, with a primary emphasis on animal-based research tissues. The clear descriptions of the methods are easily followed and the specific design charts show how to plan an experiment. Included are a series of experimental cases exploring actual mistakes that demonstrate the approaches presented. Immunocytochemistry is no longer considered a specialized technique - Immunocytochemistry: A Practical Guide for Biomedical Research showcases this mainstream morphological approach in an accessible and exciting manner.

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Produktdetails

• Verlag: Springer-Verlag GmbH
• Seitenzahl: 223
• Erscheinungstermin: 8. Dezember 2009
• Englisch
• ISBN-13: 9781441913043
• Artikelnr.: 37352748

Inhaltsangabe

Chapter 1- IntroductionChapter 2- AntibodiesChapter 3- Sample Preparation/FixationChapter 4- Tissue EmbeddingChapter 5- Blocking and PermeabilityChapter 6- Labels for AntibodiesChapter 7- Application MethodsChapter 8- ControlsChapter 9- Method and Label DecisionChapter 10- Single Antibody ProcedureChapter 11- Multiple Antibodies Different SpeciesChapter 12- Multiple Antibodies Same SpeciesChapter 13- Microscopy and ImagesChapter 14- Trouble-ShootingChapter 15- Electron Microscopic ImmunocytochemistryGlossaryProtocolsReferencesIndex