Enzymes in RNA Science and Biotechnology (eBook, ePUB)

• Format: ePub

Produktbeschreibung

Enzymes in RNA Science and Biotechnology, Volume 691 in the Methods in Enzymology series, highlights new advances in the field, including chapters on Reverse transcriptase Part I (discovery, preparation, general utilization, MarathonRT for routine RT-PCR and for cDNA synthesis on challenging RNA templates Structured RNAs, repeat RNAs and more, Engineering TNA Polymerases Through Iterative Cycles of Directed Evolution, Reverse transcriptase Part II (RNA structure mapping and determination), RNA G-quadruplex (rG4) structure detection using RTS and SHALiPE assays, tRNA Structure-seq in vivo and in droplets, Capture the in vivo intact RNA structurome by CAP-STRUCTURE-seq, and more.

• Provides the authority and expertise of leading contributors from an international board of authors
• Presents the latest release in Methods in Enzymology serials
• Updated release includes the latest information on Enzymes in RNA science and biotechnology

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Produktdetails

• Verlag: Elsevier Science & Techn.
• Seitenzahl: 412
• Erscheinungstermin: 30. Oktober 2023
• Englisch
• ISBN-13: 9780443157714
• Artikelnr.: 69249419

Inhaltsangabe

Reverse transcriptase Part I (discovery, preparation, general
utilization)1. MarathonRT for routine RT-PCR and for cDNA synthesis on
challenging RNA templates Structured RNAs, repeat RNAs and more2.
Engineering TNA Polymerases Through Iterative Cycles of Directed
EvolutionReverse transcriptase Part II (RNA structure mapping and
determination)3. RNA G-quadruplex (rG4) structure detection using RTS and
SHALiPE assays4. tRNA Structure-seq in vivo and in droplets5. Capture the
in vivo intact RNA structurome by CAP-STRUCTURE-seq6. Sequencing-based
analysis of RNA structures in living cells with SHAPE-MaPRNA polymerase
(discovery, preparation, development/engineering and application)7. Making
RNA: Using T7RNA polymerase to produce high yields of RNA from DNA
templates8. A Simple Approach to Improving RNA Synthesis: Salt Inhibition
of RNA Rebinding Coupled with Strengthening Promoter Binding by a Targeted
Gap in the DNA9. Isolation of E. coli RNA polymerase transcription
elongation complexes by selective photoreversible solid-phase
immobilizationRNA ligase10. RNA ligation using vaccinia RNA ligase enzyme:
high yield RNA coupling for RNA circularization, adapter ligation and other
applications11. Chemical crosslinking and ligation methods for in vivo
analysis of RNA structures and interactionsPost-transcriptional
modification enzymes 12. Genome-wide detection of dihydrouridine
modifications in RNA13. BID-seq: The quantitative base-resolution method
for pseudouridines in mammalian mRNA.14. Quantitative DAMM-seq maps
multiple RNA methylations in tRNA and mitochondrial dsRNA15. Discovering
RNA modification enzymes by a comparative genomics approach16. Functional
analysis of tRNA modification enzymes using Mutational profiling17.
Fluorescent labelling of tRNA for interaction studies with RNA modification
enzymes18. Synthesis of long RNA with a site-specific modification by
enzymatic splint ligationEndo/Exo nucleases 19. Characterizing RNase
L-mediated mRNA decay in single cells20. Characterization of RNase J21.
DICER cleavage assays22. 23. DROSHA cleavage assays