Chaperonin Protocols (eBook, PDF)

Redaktion: Schneider, Christine

• Format: PDF

Produktbeschreibung

In Chaperonin Protocols, Christine Schneider has assembled a unique collection of readily reproducible protocols for the study of chaperonins, intracellular proteins critical to many biological processes. Written by experienced investigators who have successfully honed their methods to a fineness, the protocols focus on the purification of chaperonins from different species along with their corresponding cofactors, and on chaperonin activity assays for in vivo as well as in vitro work. Many activity assays are given for GroEL, which can also be applied to mitochrondrial Hsp60. There are also assays for the eukaryotic chaperonin TRiC and handy methods-for example, one for preparing labeled probes-that can be used for various purposes and prove helpful in numerous different procedures. Critically important to a greater understanding of such disorders as cystic fibrosis, Alzheimer's disease, and BSE, Chaperonin Protocols offers both novice and experienced investigators fast access to today's best and most productive chaperonin methods, all explained in step-by-step detail to ensure robust and reproducible results.

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Produktdetails

• Verlag: Humana Press
• Seitenzahl: 212
• Erscheinungstermin: 5. Februar 2008
• Englisch
• ISBN-13: 9781592590612
• Artikelnr.: 69699118

Inhaltsangabe

Purification of Archaeal Chaperonin from Sulfolobus shibatae.- Purification of Hsp60 from Thermus thermophilus.- Purification of GroEL from an Overproducing E. coliStrain.- Purification of GroES from an Overproducing E. coliStrain.- Purification of the Gp31 Co-chaperonin of BacteriophageT4.- Removing Trace Fluorescent Contaminants from GroEL Preparations.- Assembly and Disassembly of GroEL and GroES Complexes.- GroEL/GroES Interaction Assayed by Protease Protection.- Determination of Chaperonin Activity In Vivo.- Interaction of Nonnative Polypeptide Substrates with the Escherichia coli Chaperonin GroEL.- Prevention of Rhodanese Aggregation by the Chaperonin GroEL.- Refolding of Bovine Mitochondrial Rhodanese by Chaperonins GroEL and GroES.- Assay of Malate Dehydrogenase.- Assay of Chaperonin-Assisted Refolding of Citrate Synthase.- Purification of Yeast Mitochondrial Hsp60.- Preparation of Recombinant Human Hsp10.- Purification of the Cytosolic ChaperoninTRiC from Bovine Testis.- Monitoring Actin Folding.- Folding Assays.- Purification of Prefoldin.- Purification of GimC from Saccharomyces cerevisiae.- Analysis of Eukaryotic Molecular Chaperone Complexes Involved in Actin Folding.

Rezensionen

"This book is highly recommended for academic libraries and for those special or industrial libraries that support a program in microbiology. Because the clear and logical approach to each protocol can serve as a model experiment that is easily reproduced, this book would be an especially valuable tool for students."-E-Streams (Electronic Reviews of Science and Technology References) "...an invaluable overview of two main aspects of chaperonin research-purification of chaperonins and their cofactors and assays to monitor folding activity. ...of excellent chapters on more diverse chaperonins, including those from Archaea and the specialized, eukaryotic TRiC complex....The book provides a wealth of explicitly detailed protocols (together with useful troubleshooting notes) for all those working in the field of chaperonins and protein folding or misfolding."...Microbiology Today