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Small GTPase binding proteins (GTPases) are an ancient group of proteins that play key roles in almost every aspect of cell biology, from cell proliferation to nuclear transport. In GTPase Protocols: The Ras Superfamily, Edward J. Manser and Thomas Leung have collected the key techniques currently in use to probe the function of these ubiquitous proteins both in vitro and in vivo. Presented in a format that ensures ready reproducibility by accomplished experimentalists who have refined the various methods in their laboratories, each technique includes step-by-step instructions, tips on avoiding pitfalls and troubleshooting, and ancillary notes explaining how to adapt each procedure in the event of problems. The methods cover the spectrum of core techniques required for the five major GTPase subfamilies (Ras, Rho, Rab, Arf, and Ran) and permit a diversity of applications ranging from structural studies on a GTPase to real time in vivo analysis. Timely and highly practical, GTPase Protocols: The Ras Superfamily illuminates the powerful techniques used by investigators today to study this special family of proteins that plays such important roles in human health and disease.
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From the reviews:
"The 20 protocols provide detailed procedures for working with most of the known small GTPases of the RAS superfamily. Included are those from RAS, RHO, RAB, ARF, and RAN. In each section, several different approaches are given, including methods for studying downstream effects. Assay methods range from microarray analysis to cell motility measurements. In each case, there is sufficient detail to allow investigators new to the procedure to use them effectively. In addition, the limitations of the procedures are provided as well as useful "notes" to facilitate adoption in the laboratory. A short bibliography accompanies each chapter and provides adequate primary source references."-Doody's Health Sciences Book Review Journal
"In GTPase Protocols, Manser and Leung assemble the key protocols required for researchers venturing into this field ... . combination of clear practical detail with underlying theory provides the reader with the information required not only to perform the technique but also to interpret the resulting data. ... In summary, this is a collection of detailed and precise experimental methods from some of the leading research groups in this field. The authors are to be congratulated on the clarity brought to each technique ... ." (Harry Mellor, Journal of Cell Science, Vol. 116 (7-8), 2003)
"The 20 protocols provide detailed procedures for working with most of the known small GTPases of the RAS superfamily. Included are those from RAS, RHO, RAB, ARF, and RAN. In each section, several different approaches are given, including methods for studying downstream effects. Assay methods range from microarray analysis to cell motility measurements. In each case, there is sufficient detail to allow investigators new to the procedure to use them effectively. In addition, the limitations of the procedures are provided as well as useful "notes" to facilitate adoption in the laboratory. A short bibliography accompanies each chapter and provides adequate primary source references."-Doody's Health Sciences Book Review Journal
"In GTPase Protocols, Manser and Leung assemble the key protocols required for researchers venturing into this field ... . combination of clear practical detail with underlying theory provides the reader with the information required not only to perform the technique but also to interpret the resulting data. ... In summary, this is a collection of detailed and precise experimental methods from some of the leading research groups in this field. The authors are to be congratulated on the clarity brought to each technique ... ." (Harry Mellor, Journal of Cell Science, Vol. 116 (7-8), 2003)