This book, written by expert scientists in the field, analyses how diverse fields of scientific research interact on a specific example - RNA polymerases.
This book, written by expert scientists in the field, analyses how diverse fields of scientific research interact on a specific example - RNA polymerases.
Professor Henri C Buc, Institut Pasteur, Paris, France has a special interest in the enzymology of nucleic acid polymerases. Terence R Strick, Centre National de la Recherche Scientifique, Paris, France has a special interest in single-molecule enzymology.
Inhaltsangabe
Chapter 1: Where it all Begins: An Overview of Promoter Recognition and Open Complex Formation; Chapter 2: Opening the DNA at the Promoter; The Energetic Challenge; Chapter 3: Intrinsic In Vivo Modulators: Negative Supercoiling and the Constituents of the Bacterial Nucleoid; Chapter 4: Transcription by RNA Polymerases: From Initiation to Elongation, Translocation and Strand Separation; Chapter 5: Single-molecule FRET Analysis of the Path from Transcription Initiation to Elongation; Chapter 6: Real-time Detection of DNA Unwinding by Escherichia coli RNAP: From Transcription Initiation to Termination; Part II: Transcription Elongation and Termination, Interlude, The Engine and the Brake; Chapter 7: Substrate Loading, Nucleotide Addition, and Translocation by RNA Polymerase; Chapter 8: Regulation of RNA Polymerase through its Active Center; Chapter 9: Kinetic Modeling of Transcription Elongation; Chapter 10: Mechanics of Transcription Termination; Conclusion: Past, Present, and Future of Single-molecule Studies of Transcription
Chapter 1: Where it all Begins: An Overview of Promoter Recognition and Open Complex Formation; Chapter 2: Opening the DNA at the Promoter; The Energetic Challenge; Chapter 3: Intrinsic In Vivo Modulators: Negative Supercoiling and the Constituents of the Bacterial Nucleoid; Chapter 4: Transcription by RNA Polymerases: From Initiation to Elongation, Translocation and Strand Separation; Chapter 5: Single-molecule FRET Analysis of the Path from Transcription Initiation to Elongation; Chapter 6: Real-time Detection of DNA Unwinding by Escherichia coli RNAP: From Transcription Initiation to Termination; Part II: Transcription Elongation and Termination, Interlude, The Engine and the Brake; Chapter 7: Substrate Loading, Nucleotide Addition, and Translocation by RNA Polymerase; Chapter 8: Regulation of RNA Polymerase through its Active Center; Chapter 9: Kinetic Modeling of Transcription Elongation; Chapter 10: Mechanics of Transcription Termination; Conclusion: Past, Present, and Future of Single-molecule Studies of Transcription
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