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In Tunisia, the use of medicinal plants is widely answered and occupies a major place in the treatment of various diseases. Our study allowed us to conclude the beneficial effects of the administration of the aqueous extract of the pomegranate in the prevention of the toxic effects of free radicals during oxidative stress. This work allowed us to reach the following conclusion: We have evaluated the antioxidant activity of the aqueous extract, furthermore, our results show that the extract of the plant (Punica granatum) causes an improvement of the antioxidant status at the hepatic level as well as the improvement of the transaminase (ASAT) and the plasma LDH. Indeed, the decrease of MDA concentration, increase of thiol group (GSH), superoxide dismutase (SOD) activity and catalase (CAT) activity at the hepatic level in rats treated with the extract show the antioxidant properties of Punica granatum. In conclusion, Punica granatum is a plant rich in therapeutic compounds.