Pseudokinases, Volume 667, the latest release in the Methods in Enzymology serial, highlights new advances in the field with this new volume presenting interesting chapters, including the Production and Purification of the PEAK pseudokinases for structural and functional studies, Structural biology and biophysical characterization of Tribbles pseudokinases, Detecting endogenous TRIB protein expression and its downstream signaling, Analysis of human Tribbles 2 pseudokinase, Expression, purification and examination of ligand-binding to IRAK pseudokinases, Characterization of pseudokinase…mehr
Pseudokinases, Volume 667, the latest release in the Methods in Enzymology serial, highlights new advances in the field with this new volume presenting interesting chapters, including the Production and Purification of the PEAK pseudokinases for structural and functional studies, Structural biology and biophysical characterization of Tribbles pseudokinases, Detecting endogenous TRIB protein expression and its downstream signaling, Analysis of human Tribbles 2 pseudokinase, Expression, purification and examination of ligand-binding to IRAK pseudokinases, Characterization of pseudokinase ILK-mediated actin assembly, Biochemical examination of Titin pseudokinase, Approaches to study pseudokinase conformations, CRISPR editing cell lines for reconstitution studies of pseudokinase function, and much more.
James Murphy is Associate Professor and the head of the Inflammation Division at the WEHI (formerly known as The Walter and Eliza Hall Institute of Medical Research) in Melbourne and is closely associated with The University of Melbourne and The Royal Melbourne Hospital. James' lab studies the protein-protein interactions that underpin signal transduction. Much of his work is focused on understanding the molecular mechanisms by which protein kinases and their relatives, pseudokinases, regulate cell signaling. Their work on the pseudokinase, MLKL, provided a template for developing a detailed understanding of how the remaining ~50 uncharacterized pseudokinases modulate cell signaling.
Inhaltsangabe
1. Production and Purification of the PEAK pseudokinases for structural and functional studies 2. Structural biology and biophysical characterisation of Tribbles pseudokinases 3. Detecting endogenous TRIB protein expression and its downstream signalling 4. Analysis of human Tribbles 2 pseudokinase 5. Expression, purification and examination of ligand-binding to IRAK pseudokinases 6. Characterization of pseudokinase ILK-mediated actin assembly 7. Biochemical examination of Titin pseudokinase 8. Approaches to study pseudokinase conformations 9. CRISPR editing cell lines for reconstitution studies of pseudokinase function 10. Proximity ligation approaches to define the interactomes of pseudokinases 11. Determining the role of the pseudokinase domain in guanylyl cyclase A and B signaling 12. Strategies to study and stimulate bacterial pseudokinases 13. Dynamics of Kinases and Pseudokinases by HDX-MS 14. In-cell thermal shift assay for drug binding analysis to receptor pseudokinases 15. Pseudoenzymology of Kinase Suppressor of Ras 16. Computational and evolutionary insights into pseudokinase mechanisms 17. Methods to identify small molecule allosteric modulators of the STRAD pseudokinase 18. Pharmacological targeting of the pseudokinase HER3 19. Considerations and analysis of the unusual pseudokinase BUBR1 20. The pseudokinase domain of receptor guanylyl cyclases: binding of ATP and allosteric regulation of guanylyl cyclase activity 21. Methods for discovering catalytic activities for pseudokinases 22. Efficient Expression, Purification, and Visualization by Cryo-EM of Fully-Glycosylated Unliganded HER3 23. An effective strategy for ligand-mediated pulldown of the HER2/HER3 receptor complex and cryo-EM structure determination at low sample concentrations 24. Identification of small molecule binders for pseudokinases 25. Identification and characterization of Tyk2-JH2 pseudokinase domain inhibitors 26. Monitoring the ATPase activity of Bud32 and its role in tRNA binding
1. Production and Purification of the PEAK pseudokinases for structural and functional studies 2. Structural biology and biophysical characterisation of Tribbles pseudokinases 3. Detecting endogenous TRIB protein expression and its downstream signalling 4. Analysis of human Tribbles 2 pseudokinase 5. Expression, purification and examination of ligand-binding to IRAK pseudokinases 6. Characterization of pseudokinase ILK-mediated actin assembly 7. Biochemical examination of Titin pseudokinase 8. Approaches to study pseudokinase conformations 9. CRISPR editing cell lines for reconstitution studies of pseudokinase function 10. Proximity ligation approaches to define the interactomes of pseudokinases 11. Determining the role of the pseudokinase domain in guanylyl cyclase A and B signaling 12. Strategies to study and stimulate bacterial pseudokinases 13. Dynamics of Kinases and Pseudokinases by HDX-MS 14. In-cell thermal shift assay for drug binding analysis to receptor pseudokinases 15. Pseudoenzymology of Kinase Suppressor of Ras 16. Computational and evolutionary insights into pseudokinase mechanisms 17. Methods to identify small molecule allosteric modulators of the STRAD pseudokinase 18. Pharmacological targeting of the pseudokinase HER3 19. Considerations and analysis of the unusual pseudokinase BUBR1 20. The pseudokinase domain of receptor guanylyl cyclases: binding of ATP and allosteric regulation of guanylyl cyclase activity 21. Methods for discovering catalytic activities for pseudokinases 22. Efficient Expression, Purification, and Visualization by Cryo-EM of Fully-Glycosylated Unliganded HER3 23. An effective strategy for ligand-mediated pulldown of the HER2/HER3 receptor complex and cryo-EM structure determination at low sample concentrations 24. Identification of small molecule binders for pseudokinases 25. Identification and characterization of Tyk2-JH2 pseudokinase domain inhibitors 26. Monitoring the ATPase activity of Bud32 and its role in tRNA binding
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