Biological functions are almost exclusively attributed to macromolecules, i.e. nucleic acids, proteins and polysaccharides. To gain their complete functional activities these biomolecules have to associate with the nuclear matrix, the cytoskeleton and the cell/plasma membranes. It is the aim of this series to discuss actual aspects in the field of structure-associated genetic and epigenetic functional processes. This series of survey reviews fills the gap in structure-associated information flow, and is a vital reference work for scientists in molecular and cell biology.
Biological functions are almost exclusively attributed to macromolecules, i.e. nucleic acids, proteins and polysaccharides. To gain their complete functional activities these biomolecules have to associate with the nuclear matrix, the cytoskeleton and the cell/plasma membranes. It is the aim of this series to discuss actual aspects in the field of structure-associated genetic and epigenetic functional processes. This series of survey reviews fills the gap in structure-associated information flow, and is a vital reference work for scientists in molecular and cell biology.
Association of DNA with Nuclear Matrix.- A. Introduction.- B. MAR Elements and Their Properties.- C. Roles of MAR Elements in DNA Packaging and DNA Replication.- References.- Nuclear Import in Vitro.- A. Overview of Nuclear Import.- B. In Vitro Systems for Studying Nuclear Import.- C. Conclusion.- References.- Cytoplasmic Assembly and Nuclear Transport of the snRNP Particles.- A. Introduction.- B. Maturation of the snRNAs.- C. snRNP Particle Assembly.- D. Nuclear Accumulation of snRNP Particles.- E. Summary and Perspectives.- References.- The Centrosome: Recent Advances on Structure and Functions.- A. Definitions.- B. Isolation of Centrosomes from Somatic Cells.- C. The Structure of Isolated Centrosomes.- D. Centrosomal Proteins.- E. The Centrosome and the Nucleation of Microtubules.- F. The Centrosome Cycle in the Cell Cycle.- G. Centrosome Continuity.- H. Centrosome and the Spindle Formation.- I. Centrosome and the Spatial Organization of Microtubules in Terminally Differentiated Cells.- J. Centrosome and Cell Polarity/Movement.- K. Conclusion.- References.- Role of Nonsense, Frameshift, and Missense Suppressor tRNAs in Mammalian Cells.- A. Introduction.- B. Suppression of Nonsense Codons.- C. Ribosomal Frameshifting.- D. Missense Suppression and Misrecognition of Genetic Codewords.- E. Conclusion.- References.- UAG Suppressor Glutamine tRNA in Uninfected and Retrovirus-Infected Mammalian Cells.- A. Introduction.- B. Isolation and Sequence Analysis of Glutamine tRNA from Mammalian Cells.- C. Analysis of Suppressor Activity of Mammalian Glutamine tRNA.- D. Selective Increase of Suppressor Glutamine tRNA in Retrovirus-Infected Cells.- E. Influence of Increased Amount of Suppressor tRNA on Translation Reaction of Cellular mRNA.- F. Discussion.- References.- Essential Genes for Development of Dictyostelium.- A. Introduction.- B. Genetic Analysis in Dictyostelium.- C. Temporal Sequence of Differentiations.- D. Initiation of Development.- E. Stage 1: Chemotaxis.- F. cAMP Regulation of Transcription and Chemotaxis.- G. Stage 2: Integration.- H. Stage 3: Divergence.- I. Stage 4: Culmination.- J. Dependent Sequence.- References.
Association of DNA with Nuclear Matrix.- A. Introduction.- B. MAR Elements and Their Properties.- C. Roles of MAR Elements in DNA Packaging and DNA Replication.- References.- Nuclear Import in Vitro.- A. Overview of Nuclear Import.- B. In Vitro Systems for Studying Nuclear Import.- C. Conclusion.- References.- Cytoplasmic Assembly and Nuclear Transport of the snRNP Particles.- A. Introduction.- B. Maturation of the snRNAs.- C. snRNP Particle Assembly.- D. Nuclear Accumulation of snRNP Particles.- E. Summary and Perspectives.- References.- The Centrosome: Recent Advances on Structure and Functions.- A. Definitions.- B. Isolation of Centrosomes from Somatic Cells.- C. The Structure of Isolated Centrosomes.- D. Centrosomal Proteins.- E. The Centrosome and the Nucleation of Microtubules.- F. The Centrosome Cycle in the Cell Cycle.- G. Centrosome Continuity.- H. Centrosome and the Spindle Formation.- I. Centrosome and the Spatial Organization of Microtubules in Terminally Differentiated Cells.- J. Centrosome and Cell Polarity/Movement.- K. Conclusion.- References.- Role of Nonsense, Frameshift, and Missense Suppressor tRNAs in Mammalian Cells.- A. Introduction.- B. Suppression of Nonsense Codons.- C. Ribosomal Frameshifting.- D. Missense Suppression and Misrecognition of Genetic Codewords.- E. Conclusion.- References.- UAG Suppressor Glutamine tRNA in Uninfected and Retrovirus-Infected Mammalian Cells.- A. Introduction.- B. Isolation and Sequence Analysis of Glutamine tRNA from Mammalian Cells.- C. Analysis of Suppressor Activity of Mammalian Glutamine tRNA.- D. Selective Increase of Suppressor Glutamine tRNA in Retrovirus-Infected Cells.- E. Influence of Increased Amount of Suppressor tRNA on Translation Reaction of Cellular mRNA.- F. Discussion.- References.- Essential Genes for Development of Dictyostelium.- A. Introduction.- B. Genetic Analysis in Dictyostelium.- C. Temporal Sequence of Differentiations.- D. Initiation of Development.- E. Stage 1: Chemotaxis.- F. cAMP Regulation of Transcription and Chemotaxis.- G. Stage 2: Integration.- H. Stage 3: Divergence.- I. Stage 4: Culmination.- J. Dependent Sequence.- References.
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