Laboratory Methods in Enzymology: Protein Part C

Herausgegeben:Lorsch, Jon

• Gebundenes Buch

Produktbeschreibung

In this volume we have brought together a number of core protocols concentrating on Protein, carefully written and edited by experts.

Produktdetails

• Methods in Enzymology Volume 541
• Verlag: Academic Press / Elsevier Science & Technology
• Artikelnr. des Verlages: C2013-0-09948-3
• Englisch
• Abmessung: 17mm x 152mm x 229mm
• Gewicht: 590g
• ISBN-13: 9780124201194
• Artikelnr.: 40444055

Inhaltsangabe

TCA PrecipitationCoimmunoprecipitation of Proteins from YeastCoupling Antibody to Cyanogen Bromide-Activated SepharoseAnalysis of Protein-Protein Interactions by CoimmunoprecipitationUse and Application of Hydrophobic Interaction Chromatography for Protein PurificationHydroxyapatite Chromatography: Purification Strategies for Recombinant ProteinsSalting out of Proteins Using Ammonium Sulfate PrecipitationUsing Ion Exchange Chromatography to Purify a Recombinantly Expressed ProteinGel Filtration Chromatography (Size Exclusion Chromatography) of ProteinsExpression and Purification of Membrane ProteinsExplanatory Chapter: Choosing the Right DetergentOne-dimensional SDS-Polyacrylamide Gel Electrophoresis (1D SDS-PAGE)Coomassie Blue StainingSilver Staining of SDS-polyacrylamide GelStandard In Vitro Assays for Protein-Nucleic Acid Interactions - Gel shift Assays for RNA and DNA BindingProtein Filter BindingExplanatory Chapter: Troubleshooting recombinant protein expression: generalExplanatory Chapter: Troubleshooting protein expression: what to do when the protein is not solubleWestern Blotting using Chemiluminescent Substrates