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Recognizing the gaps in the teaching of Science and Biology in intercultural education, this research was based on the application of a Didactic Sequence in the training of indigenous teachers, with the aim of investigating how a didactic sequence can provide the incorporation of educational technologies in the pedagogical planning of indigenous teachers as an alternative resource for working on the teaching of Science and Biology in an intercultural way. Finally, this research sought to demonstrate that the use of the Didactic Sequence proposed here, mediated by Educational Technologies and applied during the training of indigenous teachers, can help in the teaching of Science and Biology in schools in indigenous communities.