The conference represented by this book was made possible by support from NICHD and a planning committee headed by Dr. Richard Sherins. Two general areas of research are included: the first encompasses steroid hormone synthesis, metabolism and transport in the testis; and the second relates to hormonal regula tion of the seminiferous tubule with special emphasis on the con trol of Sertoli cell function. In addition, there are sections on the purification of unique testicular proteins and morpho logical studies with particular emphasis on the Sertoli cell. We would like to express our sincere…mehr
The conference represented by this book was made possible by support from NICHD and a planning committee headed by Dr. Richard Sherins. Two general areas of research are included: the first encompasses steroid hormone synthesis, metabolism and transport in the testis; and the second relates to hormonal regula tion of the seminiferous tubule with special emphasis on the con trol of Sertoli cell function. In addition, there are sections on the purification of unique testicular proteins and morpho logical studies with particular emphasis on the Sertoli cell. We would like to express our sincere thanks to Dr. Sherins and his staff at NICHD and to all of the people at the University of North Carolina who participated in the Conference arrangements, to Dr. Judson J. Van Wyk, Chief of the Pediatric Endocrinology Division, and Dr. H. Stanley Bennett, Director of the Laboratories for Reproductive Biology. Our very special thanks to Mrs. Carolyn Jaros for her help in handling the local arrangements. Mrs. Martha Byrd and Mrs. Linda Rollins typed the manuscripts. Miss Leslie Wells and Mr. Albert Smith kindly assisted in proof reading, and Dr. Elizabeth Wilson gave much help with the final editing process. To all of these people, we are most grateful.
Steroid synthesis and metabolism: in vitro studies.- 1. Distribution of steroids, steroid production and steroid metabolizing enzymes in rat testis.- 2. Androgen metabolism in the seminiferous tubule.- 3. Androgen synthesis and metabolism by preparations from the seminiferous tubule of the rat testis.- 4. Gonadotropic regulation of 5?-reductase activity in the interstitial cells and whole testis homogenate of the immature rat.- 5. Compartmentation of microsomal cytochrome P-450 and 17?-hydroxylase activity in the rat testis.- 6. Synthesis of estradiol-l7? by Sertoli cells in culture: Stimulation by FSH and dibutyryl cyclic AMP.- 7. Comparison of steroid metabolism in testicular compartments of human and rat testes.- 8. Biosynthesis and metabolism of testosterone in isolated germ cells of the rat and the ram and in the Sertoli cells of the rat.- 9. Testicular enzymes related to steroid metabolism.- Steroid synthesis and metabolism: perfusion and superfusion studies.- 10. Perfusion of the male rat reproductive tract: A model for the study of androgen secretion and action.- 11. Some factors affecting testosterone, dihydrotestosterone, 5?-androstan-3?, 17?-diol and 5?-androstan-3?, 17?-diol secretion by in vitro perfused rabbit testes.- 12. Metabolism and secretion of androgens by rat seminiferous tubules: Tracer superfusion studies.- 13. Local increase in concentration of steroids by venous-arterial transfer in the pampiniform plexus.- Steroid hormones in the seminiferous tubule.- 14. Regulation of testosterone and dihydrotestosterone levels in rete testis fluid. Evidence for androgen biosynthesis in seminiferous tubules in vivo.- 15. Concentration of steroid hormones and androgen binding protein (ABP) in rabbit efferent duct fluid.- 16. The blood - testisbarrier and steroids.- Androgen receptors.- 17. Physicochemical and biological properties of androgen receptors.- 18. Androgen receptor in rat testis.- 19. Localization of androgen receptors in rat testis: Biochemical studies.- 20. Androgen receptors in testis tissue enriched in Sertoli cells.- 21. Androgen binding in the testis: In vitro production of androgen binding protein (ABP) by Sertoli cell cultures and measurement of nuclear bound androgen by a nuclear exchange assay.- 22. Localization of androgen target cells in the rat testis: Autoradiographic studies.- Hormonal regulation of Sertoli cell function.- 23. Testicular androgen binding protein (ABP) - A parameter of Sertoli cell secretory function.- 24. Hormonal regulation of the seminiferous tubule Function.- 25. Invitro synthesis of testicular androgen binding protein (ABP): Stimulation by FSH and androgen.- 26. Biochemical responses of cultured Sertoli cell-enriched preparations to follicle stimulating hormone and dibutyryl cyclic AMP.- 27. FSH-induction of androgen binding protein in testes of Sertoli cell-only rats.- 28 Culture and FSH responses of Sertoli cells isolated from sexually mature rat testis.- 29 Stimulation by follicle stimulating hormone and dibutyryl cyclic AMP of incorporation of 3H-thymidine into nuclear DNA of cultured Sertoli cell-enriched preparations from immature rats.- Purification of testicular proteins.- 30. On three varieties of specific basic proteins associated with mammalian spermatogenesis.- 31. Purification and characterization of rabbit testicular androgen binding protein (ABP).- Morphological studies on the seminiferous tubule.- 32. Transcription during mammalian spermatogenesis with special reference to Sertoli cells.- 33. Histochemical and ultrastructural observations onnormal and follicle stimulating hormone-injected prepuberal rat Sertoli cells.- 34. Follicle stimulating hormone and cyclic AMP directed changes in ultrastructure and properties of cultured Sertoli cell-enriched preparations: Comparison with cultured testicular peritubular cells.- 35. Possible boundary tissue function in isolated seminiferous tubules.- 36. Conference Summary.
Steroid synthesis and metabolism: in vitro studies.- 1. Distribution of steroids, steroid production and steroid metabolizing enzymes in rat testis.- 2. Androgen metabolism in the seminiferous tubule.- 3. Androgen synthesis and metabolism by preparations from the seminiferous tubule of the rat testis.- 4. Gonadotropic regulation of 5?-reductase activity in the interstitial cells and whole testis homogenate of the immature rat.- 5. Compartmentation of microsomal cytochrome P-450 and 17?-hydroxylase activity in the rat testis.- 6. Synthesis of estradiol-l7? by Sertoli cells in culture: Stimulation by FSH and dibutyryl cyclic AMP.- 7. Comparison of steroid metabolism in testicular compartments of human and rat testes.- 8. Biosynthesis and metabolism of testosterone in isolated germ cells of the rat and the ram and in the Sertoli cells of the rat.- 9. Testicular enzymes related to steroid metabolism.- Steroid synthesis and metabolism: perfusion and superfusion studies.- 10. Perfusion of the male rat reproductive tract: A model for the study of androgen secretion and action.- 11. Some factors affecting testosterone, dihydrotestosterone, 5?-androstan-3?, 17?-diol and 5?-androstan-3?, 17?-diol secretion by in vitro perfused rabbit testes.- 12. Metabolism and secretion of androgens by rat seminiferous tubules: Tracer superfusion studies.- 13. Local increase in concentration of steroids by venous-arterial transfer in the pampiniform plexus.- Steroid hormones in the seminiferous tubule.- 14. Regulation of testosterone and dihydrotestosterone levels in rete testis fluid. Evidence for androgen biosynthesis in seminiferous tubules in vivo.- 15. Concentration of steroid hormones and androgen binding protein (ABP) in rabbit efferent duct fluid.- 16. The blood - testisbarrier and steroids.- Androgen receptors.- 17. Physicochemical and biological properties of androgen receptors.- 18. Androgen receptor in rat testis.- 19. Localization of androgen receptors in rat testis: Biochemical studies.- 20. Androgen receptors in testis tissue enriched in Sertoli cells.- 21. Androgen binding in the testis: In vitro production of androgen binding protein (ABP) by Sertoli cell cultures and measurement of nuclear bound androgen by a nuclear exchange assay.- 22. Localization of androgen target cells in the rat testis: Autoradiographic studies.- Hormonal regulation of Sertoli cell function.- 23. Testicular androgen binding protein (ABP) - A parameter of Sertoli cell secretory function.- 24. Hormonal regulation of the seminiferous tubule Function.- 25. Invitro synthesis of testicular androgen binding protein (ABP): Stimulation by FSH and androgen.- 26. Biochemical responses of cultured Sertoli cell-enriched preparations to follicle stimulating hormone and dibutyryl cyclic AMP.- 27. FSH-induction of androgen binding protein in testes of Sertoli cell-only rats.- 28 Culture and FSH responses of Sertoli cells isolated from sexually mature rat testis.- 29 Stimulation by follicle stimulating hormone and dibutyryl cyclic AMP of incorporation of 3H-thymidine into nuclear DNA of cultured Sertoli cell-enriched preparations from immature rats.- Purification of testicular proteins.- 30. On three varieties of specific basic proteins associated with mammalian spermatogenesis.- 31. Purification and characterization of rabbit testicular androgen binding protein (ABP).- Morphological studies on the seminiferous tubule.- 32. Transcription during mammalian spermatogenesis with special reference to Sertoli cells.- 33. Histochemical and ultrastructural observations onnormal and follicle stimulating hormone-injected prepuberal rat Sertoli cells.- 34. Follicle stimulating hormone and cyclic AMP directed changes in ultrastructure and properties of cultured Sertoli cell-enriched preparations: Comparison with cultured testicular peritubular cells.- 35. Possible boundary tissue function in isolated seminiferous tubules.- 36. Conference Summary.
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