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Diabetes is a disorder of carbohydrate, fat & protein metabolism attributed to diminished production of Insulin or mounting resistance to its action. Probiotic and plant extract both have a great potential in terms of their beneficial effect. To control hyperglycaemia, combination of Insulin plant leaves & alternative modalities of treatment should be regularly consumed as it will avoid diabetic complications and also fewer the side effects of Insulin. Therefore, treating diabetes by natural resources appears to be a promising strategy and should be preferred for inhibiting alpha- amylase activity. In this study, two plants were selected namely, Gymnema sylvestre and Pterocarpus marsupium for their anti- diabetic properties and probiotics are isolated and allowed to homogenized with plant extracts. Three best isolated probiotics were obtained (HMP 2 HMP 4 HMP 9) among this, the combination of methanolic extract of P. marsupium with HMP 9 gives the optimum percent inhibition with 68.56%.The comparison were shown between both the plants and it is concluded that P.marsupium have the good potential effects than Gymnema sylvestre plant in controlling diabetes.