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The development of accurate and reliable security systems is a matter of wide interest, and in this context biometrics has seen as a highly effective automatic mechanism for personal authentication. Many research groups are working on the development of the different authentication systems such as the fingerprint, iris, hand, palm print etc. by using biometrics. The palm print is the first defining characteristics and natural method to be used for recognition. Moreover, ease acceptance and non invasive nature as compared to other biometric traits make it a preferable trait to be used for authentication.In this book, an automated approach to palm print recognition has been developed and discussed. Particular emphasis has been given to the alignment method, for which a new alignment algorithm has been proposed. The proposed system is reliable and user friendly as it is developed in LabVIEW 6i. The availability of datalog files in LabVIEW makes it one of the most promising candidatefor its usage as a database.