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This Special Issue aims to provide the reader with the latest Biomedical imaging innovation used to better understand the heart's physiology, performance, function, and structure. Furthermore, each imaging technique provides specific benefits for diagnosing, treating, and following cardiovascular diseases. Technological progress improves the precision, sensitivity, and accuracy of the specialized measurements needed to assess complex conditions. In this Special Issue, "Biomedical Imaging Technologies for Cardiovascular Disease-Volume II". Seventeen manuscripts were taken into consideration. All of which underwent a rigorous peer-review process. A total of eleven original articles and six reviews were published.