Hafsa Ahmad, Anil Dwivedi, Sarvesh Paliwal

Analytical studies on a standardized extract of Withania somnifera L.

• Broschiertes Buch

Produktbeschreibung

Withania somnifera Dunal (Ashwagandha) is an Indian medicinal plant with significant pharmacological properties like adaptogenic, antioxidant, hypoglycemic and anti-stroke effects. Several of its chemotypes includes NMITLI-101, NMITLI-118, NMITLI-128. The present work elaborates phytochemical and analytical studies on NMITLI118RT+ a candidate drug under the CSIR umbrella (derived from a new chemotype of W. somnifera). Here we report the development validation and applications of a simple, rapid and reliable isocratic HPLC method for quantification of Withanolide A in NMITLI118RT+ on a CN column utilizing gliclazide as an internal standard which could detect Withanolide A at about 3.8 min and gliclazide (internal standard) at about 4.8 min with a linearity range of 2-20 µg/mL. A second method here reports the development and validation of a simple isocratic method for simultaneous estimation of Withanolide A and Withanone (sesquiterpene lactone markers) established on a C-18; 3µ column which could detect Withanolide A and Withanone at retention times of about 20.7 min and 21.6 min respectively in the linearity range of 2-50 µg/mL for both.

Produktdetails

• Verlag: LAP Lambert Academic Publishing
• Seitenzahl: 52
• Erscheinungstermin: 26. Juni 2017
• Englisch
• Abmessung: 220mm x 150mm x 4mm
• Gewicht: 96g
• ISBN-13: 9783330343207
• ISBN-10: 3330343206
• Artikelnr.: 48781240

Autorenporträt

Ms. Hafsa Ahmad did her B.Pharm.& M.Pharm.(Pharmacognosy)in 2009 & 2011 from prestigious BBDNITM(presently BBD University),Lucknow,India. Her Research work entitled 'Standardization and Pharmacological Studies on Michelia champaca L.'was carried out under the guidance of Prof. Rajiv Gupta,Dean,School of Pharmacy,BBDU.