Molecular Biology in Cellular Pathology (eBook, PDF)

Redaktion: Crocker, John; Murray, Paul G.

• Format: PDF

Produktbeschreibung

The latest edition of this highly successful text, covers the major advances in the methods used in cellular and molecular pathology. In recent years, knowledge of the molecular organization of the cell has led to the development of powerful new techniques that bring greater accuracy and objectives to the diagnosis, prognosis and management of many diseases and to the study of pathological states. This book describes the latest molecular techniques available for the analysis of diseases. In particular it includes new techniques using fluorescent dyes, DNA microarrays, protein chemistry, and mass spectrometry. It also incorporates information from the Human Genome Project, and the new disciplines of genomics and proteomics, where relevant to pathology. Color plates are a new feature of this edition, illustrating the advances in fluorescence labeling of cells.

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Produktdetails

• Verlag: John Wiley & Sons
• Seitenzahl: 400
• Erscheinungstermin: 30. Juli 2003
• Englisch
• ISBN-13: 9780470864982
• Artikelnr.: 37299340

Autorenporträt

John Crocker is the editor of Molecular Biology in Cellular Pathology, published by Wiley. Paul G. Murray is the editor of Molecular Biology in Cellular Pathology, published by Wiley.

Inhaltsangabe

Preface Preface to Molecular Biology in Histopathology Contributors
BLOTTING TECHNIQUES: METHODOLOGY AND APPLICATIONS Introduction Blotting
techniques References IN SITU HYBRIDISATION IN HISTOPATHOLOGY Introduction
Experimental conditions Probes and labels Controls and pitfalls
Double-labelling Increasing the sensitivity of ISH What we do in our
laboratories Applications of ISH: examples Perspective References DNA FLOW
CYTOMETRY Introduction Definitions and terms Dye used for DNA analysis
Sample preparation for DNA analysis Analysis of the DNA histogram Quality
control Computer analysis of the DNA histogram Multiparametric measurement
Acknowledgements References INTERPHASE CYTOGENICS Introduction Interphase
cytogenics Applications Conclusion References ONCOGENES Introduction
Identification of the oncogenes Functions of the oncogenes Mechanism of
oncogene activation Oncogenes in colorectal cancer Oncogenes in breast
cancer Oncogenes in lung cancer Oncogenes in haematological malignancies
Other cancers Conclusion References MOLECULAR AND IMMUNOLOGICAL ASPECTS OF
CELL PROLIFERATION The cell cycle and its importance in clinical pathology
Molecular control of the cell cycle Cell cycle control The cell cycle and
cancer Immunocytochemical markers of proliferating cells References Further
reading INTERPHASE NUCLEOLAR ORGANISER REGIONS IN TUMOUR PATHOLOGY
Introduction The AgNORs NOR silver-staining Quantitative AgNOR analysis
AgNORs as a parameter of the level of cell proliferation Application of the
AgNOR technique to tumour pathology What future for AGNORs in tumour
pathology References APOPTOSIS AND CELL SENESCENCE Introduction Apoptosis
Cell senescence Summary References THE POLYMERASE CHAIN REACTION
Introduction Principles Analysis of products RT-PCR Quantitative PCR DNA
and RNA extraction Correlation of the PCR with morphology Problems
Applications Diagnostic applications Infectious diseases Identity The
future References Online information LASER CAPTURE MICRODISSECTION:
TECHNIQUES AND APPLICATIONS IN THE MOLECULAR ANALYSIS OF THE CANCER CELL
Introduction The principle of LCM Technical considerations Advantages and
disadvantages of LCM Applications of LCM Future perspectives
Acknowledgements References THE IN SITU POLYMERASE CHAIN REACTION
Introduction Overview of the methodology In-cell PCR technologies In-cell
amplification of DNA In-cell amplification of RNA References TAQMAN(R)
TECHNOLOGY AND REAL-TIME POLYMERASE CHAIN REACTION Introduction Probe
technologies TaqMan(r) probe and chemistry TaqMan(r) PCR conditions
Interpretation of results Relative quantitation References GENE EXPRESSION
ANALYSIS USING MICROARRAYS Introduction Microarray experiments Data
analysis Recent examples of microarray applications Conclusions
Acknowledgements References Further reading Useful websites COMPARATIVE
GENOMIC HYBRIDISATION IN PATHOLOGY Introduction Technique Data analysis
Applications Clinical applications Screening for chromosomal abnormalities
in fetal and neofetal genomes Future perspectives References DNA SEQUENCING
AND THE HUMAN GENOME PROJECT Introduction DNA sequencing: the basics
Applications of DNA sequencing The Human Genome Project Further reading
Useful websites MONOCLONAL ANTIBODIES: THE GENERATION AND APPLICATION OF
'TOOLS OF THE TRADE' WITHIN BIOMEDICAL SCIENCE Introduction Antibodies and
antigens Polyclonal antibodies Monoclonal antibody development Monoclonal
antibody variants Monoclonal antibody applications Specific applications
Therapy Specific applications Conclusions Acknowledgements References
PROTEOMICS Introduction Definitions and applications Stages in proteome
analysis Future directions References Index