Force Microscopy (eBook, PDF)

Applications in Biology and Medicine
Redaktion: Jena, Bhanu P.; Hoerber, Johann Karl H.

• Format: PDF

Produktbeschreibung

A complete examination of the uses of the atomic force microscope in biology and medicine This cutting-edge text, written by a team of leading experts, is the first detailed examination of the latest, most powerful scanning probe microscope, the atomic force microscope (AFM). Using the AFM, in combination with conventional tools and techniques, readers gain a profound understanding of the cell, subcellular organelles, and biomolecular structure and function. The text begins with three chapters describing the molecular machinery and mechanism of cell secretion and membrane fusion in cells, using approaches that combine AFM, electron microscopy, X-ray diffraction, photon correlation spectroscopy, molecular biology, biochemistry, and electrophysiology. The discovery of a new cellular structure the "porosome" or fusion pore--the cells secretory machinery, the molecular mechanism of membrane fusion in cells, and the expulsion of intravesicular contents during cell secretion are outlined in the first three chapters. The book also covers: * Identification of the "porosome" in the growth hormone secreting cell of the pituitary gland * Probing the structural and physical properties of microbial cell surfaces * Scanning probe microscopic characterization of the higher plant cell wall and its components * Case studies of nano drug delivery systems using engineered dendrimers * AFM techniques for studying living cells * Investigating the intermolecular forces of leukocyte adhesion molecules * Protein-protein interactions * Micromechanical properties of lipid bilayers and vesicles The text concludes with four chapters that examine new and emerging approaches in the use of force microscopy in biology and medicine. This text is ideal for advanced undergraduate and graduate students and researchers in cell and molecular biology, genetics, genomics, physiology, neuroscience, biophysics, and biochemistry. Not only does it provide the theory, but also practical considerations such as the selection of the right tools and approach.

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Produktdetails

• Verlag: John Wiley & Sons
• Seitenzahl: 310
• Erscheinungstermin: 12. Juli 2006
• Englisch
• ISBN-13: 9780470007693
• Artikelnr.: 37289511

Autorenporträt

BHANU P. JENA, PHD, DSc, is the George E. Palade University Professor and Distinguished Professor of Physiology at Wayne State University School of Medicine, Detroit, Michigan. In addition, Dr. Jena is Director of the University's NanoBioScience Institute. J. K. HEINRICH HÖRBER, PhD, is Professor in the Department of Physics at University of Bristol, Bristol, UK.

Inhaltsangabe

Preface. Contributors. Chapter 1. Porosome: The Universal Secretory
Machinery in Cells (Bhanu P. Jena). Chapter 2. Molecular Mechanism of
SNARE-Induced Membrane Fusion (Bhanu P. Jena). Chapter 3. Molecular
Mechanism of Secretory Vesicle Content Expulsion During Cell Secretion
(Bhanu P. Jena). Chapter 4. Fusion Pores in Growth-Hormone-Secreting Cells
of the Pituitary Gland: An AFM Study (Lloyd L. Anderson and Bhanu P. Jena).
Chapter 5. Properties of Microbial Cell Surfaces Examined by Atomic Force
Microscopy (Yves F. Dufrene). Chapter 6. Scanning Probe Microscopy of Plant
Cell Wall and Its Constituents (Ksenija Radotic, Miodrag Micic, and Milorad
Jeremic). Chapter 7. Cellular Interactions of Nano Drug Delivery Systems
(Rangaramanujam M. Kannan, Omathanu Pillai Perumal, and Sujatha Kannan).
Chapter 8. Adapting AFM Techniques for Studies on Living Cells (J. K.
Heinrich Hörber) Chapter 9. Intermolecular Forces of Leukocyte Adhesion
Molecules (Xiaohui Zhang and Vincent T. Moy). Chapter 10. Mechanisms of
Avidity Modulation in Leukocyte Adhesion Studied by AFM (Ewa P.
Wojcikiewicz and Vincent T. Moy). Chapter 11. Resolving the Thickness and
Micromechanical Properties of Lipid Bilayers and Vesicles Using AFM
(Guangzhao Mao and Xuemei Liang). Chapter 12. Imaging Soft Surfaces by SFM
(Andreas Janke and Tilo Pompe). Chapter 13. High-Speed Atomic Force
Microscopy of Biomolecules in Motion (Tilman E. Schäffer). Chapter 14.
Atomic Force Microscopy in Cytogenetics (S. Thalhammer and W. M. Heckl).
Chapter 15. Atomic Force Microscopy in the Study of Macromolecular
Interactions in Hemostasis and Thrombosis: Utility for Investigation of the
Antiphospholipid Syndrome (William J. Montigny, Anthony S. Quinn, Xiao-Xuan
Wu, Edwin G. Bovill, Jacob H. Rand, and Douglas J. Taatjes). Index