Amanda Capes-Davis, R. Ian Freshney

Freshney's Culture of Animal Cells (eBook, PDF)

A Manual of Basic Technique and Specialized Applications

• Format: PDF

Produktbeschreibung

FRESHNEY'S CULTURE OF ANIMAL CELLS THE NEW EDITION OF THE LEADING TEXT ON THE BASIC METHODOLOGY OF CELL CULTURE, FULLY UPDATED TO REFLECT NEW APPLICATIONS INCLUDING IPSCS, CRISPR, AND ORGAN-ON-CHIP TECHNOLOGIES Freshney's Culture of Animal Cells is the most comprehensive and up-to-date resource on the principles, techniques, equipment, and applications in the field of cell and tissue culture. Explaining both how to do tissue culture and why a technique is done in a particular way, this classic text covers the biology of cultured cells, how to select media and substrates, regulatory requirements, laboratory protocols, aseptic technique, experimental manipulation of animal cells, and much more. The eighth edition contains extensively revised material that reflects the latest techniques and emerging applications in cell culture, such as the use of CRISPR/Cas9 for gene editing and the adoption of chemically defined conditions for stem cell culture. A brand-new chapter examines the origin and evolution of cell lines, joined by a dedicated chapter on irreproducible research, its causes, and the importance of reproducibility and good cell culture practice. Throughout the book, updated chapters and protocols cover topics including live-cell imaging, 3D culture, scale-up and automation, microfluidics, high-throughput screening, and toxicity testing. This landmark text: * Provides comprehensive single-volume coverage of basic skills and protocols, specialized techniques and applications, and new and emerging developments in the field * Covers every essential area of animal cell culture, including lab design, disaster and contingency planning, safety, bioethics, media preparation, primary culture, mycoplasma and authentication testing, cell line characterization and cryopreservation, training, and troubleshooting * Features a wealth of new content including protocols for gene delivery, iPSC generation and culture, and tumor spheroid formation * Includes an updated and expanded companion website containing figures, artwork, and supplementary protocols to download and print The eighth edition of Freshney's Culture of Animal Cells is an indispensable volume for anyone involved in the field, including undergraduate and graduate students, clinical and biopharmaceutical researchers, bioengineers, academic research scientists, and managers, technicians, and trainees working in cell biology, molecular biology, and genetics laboratories.

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Produktdetails

• Verlag: John Wiley & Sons
• Seitenzahl: 832
• Erscheinungstermin: 17. Februar 2021
• Englisch
• ISBN-13: 9781119513032
• Artikelnr.: 61212868

Autorenporträt

AMANDA CAPES-DAVIS, PHD, is a cell culture scientist and technical writer. She was Founding Manager and Honorary Scientist at CellBank Australia, Children's Medical Research Institute (CMRI), and is a member of the International Cell Line Authentication Committee (ICLAC). She was a Reviewing Editor for the 7th edition of Culture of Animal Cells, and has written numerous journal articles, policies, protocols, and white papers on good cell culture practice. R. IAN FRESHNEY, PHD, was an honorary Senior Research Fellow at the Institute of Cancer Sciences at the University of Glasgow, UK. Dr Freshney, who died in 2019, was a world-renowned cancer biologist and a pioneer in cell culture techniques who made important contributions to new approaches for treating cancer patients. He taught cell culture courses at national and international level, and wrote and edited numerous books, including the first seven editions of Culture of Animal Cells.

Inhaltsangabe

Foreword xix

Acknowledgments xxi

Abbreviations xxiii

Book Navigation xxix

Part I Understanding Cell Culture 1

1. Introduction 3

1.1 Terminology 3

1.2 Historical Development 4

1.3 Applications 12

1.4 Advantages of Tissue Culture 13

1.5 Limitations of Tissue Culture 15

References 18

2. Biology of Cultured Cells 23

2.1 The Culture Environment 23

2.2 Cell Adhesion 23

2.3 Cell Division 28

2.4 Cell Fate 30

2.5 Cell Death 35

References 36

3. Origin and Evolution of Cultured Cells 39

3.1 Origin of Cultured Cells 39

3.2 Evolution of Cell Lines 40

3.3 Changes in Genotype 43

3.4 Changes in Phenotype 46

3.5 Senescence and Immortalization 48

Minireview M3.1 Senescence and Immortalization 48

3.6 Transformation 50

3.7 Conclusions: Origin and Evolution 58

References 58

Part II Laboratory and Regulatory Requirements 63

4. Laboratory Design and Layout 65

4.1 Design Requirements 65

4.2 Layout of Laboratory Areas 74

4.3 Disaster and Contingency Planning 80

References 83

5. Equipment and Materials 85

5.1 Sterile Handling Area Equipment 85

5.2 Imaging and Analysis Equipment 97

5.3 Incubation Equipment 99

5.4 Preparation and Washup Equipment 104

5.5 Cold Storage Equipment 107

References 109

6. Safety and Bioethics 111

6.1 Laboratory Safety 111

6.2 Hazards in Tissue Culture Laboratories 117

6.3 Biosafety 121

6.4 Bioethics 129

References 132

7. Reproducibility and Good Cell Culture Practice 137

7.1 Reproducibility 137

7.2 Good Practice Requirements 141

7.3 Cell Line Provenance 145

7.4 Validation Testing 146

7.5 Quality Assurance (QA) 148

7.6 Replicate Sampling 150

References 151

Part III Medium and Substrate Requirements 155

8. Culture Vessels and Substrates 157

8.1 Attachment and Growth Requirements 157

8.2 Substrate Materials 158

8.3 Substrate Treatments 159

8.4 Feeder Layers 163

8.5 Choice of Culture Vessel 164

8.6 Application-Specific Vessels 170

References 173

9. Defined Media and Supplements 177

9.1 Medium Development 177

9.2 Physicochemical Properties 177

9.3 Balanced Salt Solutions 185

9.4 Media Formulations 186

9.5 Serum 189

9.6 Other Media Supplements 191

9.7 Choice of Complete Medium 191

9.8 Storage of Medium and Serum 194

Suppliers 194

References 194

10. Serum-Free Media 199

10.1 Rationale for Serum-Free Medium 199

10.2 Development of Serum-Free Medium 201

10.3 Serum-Free Media Formulations 202

10.4 Serum-Free Supplements 203

10.5 Serum Replacements 209

10.6 Use of Serum-Free Medium 209

10.7 Xeno-Free Media 213

10.8 Animal Product-Free Media 214

10.9 Conclusions: Serum-Free Media 214

Suppliers 214

References 215

11. Preparation and Sterilization 219

11.1 Terminology: Preparation 219

11.2 Sterilization Methods 220

11.3 Glassware 224

Protocol P11.1 Preparation and Sterilization of Glassware 224

11.4 Other Laboratory Apparatus 229

11.5 Water 22