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This laboratory guide for successful electrophoretic separations is divided into two parts to provide readers with a thorough presentation of the fundamentals followed by a detailed description of the most common methods currently in use. This fourth edition retains the successful concept of its predecessors, yet features a brand-new layout, and is further enhanced by a section on difference gel electrophoresis, while the chapter on proteome analysis is practically all new and considerably extended, plus there are now around 10 % new literature references.
This laboratory guide for successful electrophoretic separations is divided into two parts to provide readers with a thorough presentation of the fundamentals followed by a detailed description of the most common methods currently in use. This fourth edition retains the successful concept of its predecessors, yet features a brand-new layout, and is further enhanced by a section on difference gel electrophoresis, while the chapter on proteome analysis is practically all new and considerably extended, plus there are now around 10 % new literature references.
Born in 1951, Reiner Westermeier gained his diploma in engineering in 1976, and his doctorate in 1981 at the Technical University of Munich, Germany. He remained at the TU engaged in the development of new electrophoresis methods and applications until 1983, a period that also involved several international collaborations and lecturing tours. From 1984 to 1987 Dr. Westermeier was employed as an electrophoresis products and applications specialist at LKB Instrument GmbH thereafter until 1990 at Pharmacia Biotech. He founded ETC Elektrophorese-Technik in 1991 for the development of new electrophoresis methods, media, and equipment. From January 1996 to July 1997 he was manager of scientific support Europe at Pharmacia Biotech in Freiburg, Germany. He has been at Amersham Pharmacia Biotech since July 1997, and its Senior Scientist for Proteomics since January 2002. Reiner Westermeier is the author of several publications and two books, both published by WILEY-VCH.
Inhaltsangabe
PART I: FUNDAMENTALS Introduction Electrophoresis Isotachophoresis Isoelectric Focusing Blotting Interpretation of Electropherograms Proteome Analysis Instrumentation PART II: EQUIPMENT AND METHODS Equipment for Part II Method 1: PAGE of Dyes Method 2: Agarose and Immuno Electrophoresis Method 3: Titration Curve Analysis Method 4: Native PAGE in Amphoteric Buffers Method 5: Agarose IEF Method 6: PAGIEF in Rehydrated Gels Method 7: Horizontal SDS-PAGE Method 8: Vertical PAGE Method 9: Semi-dry Blotting of Proteins Method 10: IEF in Immobilized pH Gradients Method 11: High-resolution 2D Electrophoresis Method 12: PAGE of Double Stranded DNA Method 13: Native PAGE of Single Stranded DNA Method 14: Denaturing Gradient Gel Electrophoresis Method 15: Denaturing PAGE of DNA Appendix A: Trouble-shooting References Index
