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This issue should serve as a primer to endoscopists who are seeking state-of-the-art clinical guidance on endoscopic ultrasound tissue acquisition. Authors address the changing paradigm in EUS-guided tissue acquisition and when does the oncologist require core tissue? Articles offer a comprehensive look at the core topics, including definitions in tissue acquisition, selection of FNA needles, and techniques for EUS-guides FNA and FNB. Expert authors also give their recommendations for overcoming technical challenges in EUS-guided tissue acquisition and what the pitfalls are. Readers will have a full understanding of EUS-guided tissue acquisition as well as the future directions.