Synthetic Biology, Part A (eBook, PDF)

Methods for Part/Device Characterization and Chassis Engineering
Redaktion: Voigt, Chris

• Format: PDF

Produktbeschreibung

Synthetic biology encompasses a variety of different approaches, methodologies and disciplines, and many different definitions exist. This Volume of Methods in Enzymology has been split into 2 Parts and covers topics such as Measuring and Engineering Central Dogma Processes, Mathematical and Computational Methods and Next-Generation DNA Assembly and Manipulation.

• Encompasses a variety of different approaches, methodologies and disciplines
• Split into 2 parts and covers topics such as measuring and engineering central dogma processes, mathematical and computational methods and next-generation DNA assembly and manipulation

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Produktdetails

• Verlag: Elsevier Science & Techn.
• Seitenzahl: 714
• Erscheinungstermin: 8. Juli 2011
• Englisch
• ISBN-13: 9780123850768
• Artikelnr.: 38014456

Inhaltsangabe

Section I. Measuring and Engineering Central Dogma Processes

1. Sequence-specificity and Energy Landscapes of DNA-binding Molecules


Joshua R. Tietjen, Leslie J. Donato, Devesh Bhimisaria and Aseem Z. Ansari


2. Promoter Reliability in Modular Transcriptional Networks


Rajat Anand, Navneet Rai and Mukund Thattai


3. The Analysis of ChIP-seq Data


Wenxiu Ma and Wing Hung Wong


4. Using DNA Microarrays to Assay Part Function


Virgil A. Rhodius and Carol A. Gross


5. Orthogonal gene expression in Escherichia coli


Wenlin An and Jason Chin


6. Directed evolution of promoters and tandem gene arrays for customizing RNA synthesis rates and regulation


Keith E.J. Tyo, Elke Nevoigt and Gregory Stephanopoulos

Section II. Device and System Design, Optimization, and Debugging


7. Design and Connection of Robust Genetic Circuits


Ron Weiss


8. Engineering RNAi circuits


Yaakov Benenson


9. From SELEX to cell: Dual selections for synthetic riboswitches


Joy Sinha, Shana Topp, and Justin P. Gallivan


10. Using noisy gene expression mediated by engineered adenovirus to probe signaling dynamics in mammalian cells


Jeffrey V. Wong, Guang Yao, Joseph R. Nevins and Lingchong You


11. De novo design and construction of an inducible gene expression system in mammalian cells


Maria Karlsson, Wilfried Weber and Martin Fussenegger


12. BioBuilding: Using banana-scented bacteria to teach synthetic biology


James Dixon and Natalie Kuldell

Section III. Device Measurement, Optimization, and Debugging


13. Use of Fluorescence Microscopy to Analyze Genetic Circuit Dynamics


Gürol Süel


14. Microfluidics for synthetic biology: From design to execution


M. S. Ferry, I. A. Razinkov, J. Hasty


15. Plate-based assays for light-regulated gene expression systems


Jeffrey J. Tabor


16. Spatiotemporal control of small GTPases with light using the LOV domain


Yi I. Wu, Xiaobo Wang, Li He, Denise Montell and Klaus M. Hahn


17. Light Control of Plasma Membrane Recruitment using the Phy-PIF system


Jared E. Toettcher, Delquin Gong, Wendell A. Lim, Orion D. Weiner


18. Synthetic Physiology: Strategies for Adapting Tools from Nature for Genetically-Targeted Control of Fast Biological Processes


Ed Boyden

Section IV. Devices for Metabolic Engineering


19. Metabolic Pathway Flux Enhancement by Synthetic Protein Scaffolding


John E. Dueber and Weston R. Whitaker


20. A Synthetic Iterative Pathway for ketoacid elongation


C. R. Shen, J. C. Liao

Section V. Expanding Chasses


21. Synthetic Biology in Streptomyces bacteria


Marnix Medema, Rainer Breitling and Eriko Takano


22. Methods for Engineering Sulfate Reducing Bacteria of the Genus Desulfovibrio


Kimberly L. Keller, Judy D. Wall and Swapnil Chhabra


23. Modification of the Genome of Rhodobacter sphaeroides and Construction of Synthetic Operons


Paul R. Jaschke, Rafael G. Saer, Stephan Noll and J. Thomas Beatty


24. Synthetic Biology in Cyanobacteria: Engineering and Analyzing Novel Functions


Thorsten Heidorn, Daniel Camsund, Hsin-Ho Huang, Pia Lindberg, Paulo Oliveira, Karin Stensjö, and Peter Lindblad


25. Developing a Synthetic Signal Transduction System in Plants


Kevin J. Morey, Mauricio S. Antunes, Kirk D. Albrecht, Tessa A. Bowen, Jared F. Troupe, Keira L. Havens and June I. Medford


26. Lentiviral Vectors to Study Stochastic Noise in Gene Expression

Kate Franz, Abhyudai Singh, and Leor S. Weinberger