Stem Cell Laboratory Techniques (eBook, ePUB)

A Guide for Researchers and Students
Redaktion: Subbiah, Suresh Kumar; Mok, Pooi Ling; Higuchi, Akon

• Format: ePub

Produktbeschreibung

Stem Cell Laboratory Techniques: A Guide for Researchers and Students introduces the reader to stem cell culture, handling techniques and versatile applications used by researchers. Sections introduce stem cells, including definitions, types and basic use of stem cells in biomedical science research. The book explains laboratory procedures and techniques ranging from the extraction of stem cells from animals, cell seeding and culture, harvest and maintenance of stem cells, stem cell characterization, accurate recording, quality control, and more. In addition, it guides researchers on topics such as transcriptome analysis, proliferation study analysis, and microphysiological study.

Final sections cover useful and recent applications in stem cells, such as gene editing techniques and the preparation of stem cells for in vitro study, as well as stem cell lab design and equipment used in the lab. Lastly, human and animal research ethics are discussed.

• Introduces readers to the stem cell culture and moves to handling techniques and versatile applications
• Includes coverage of gene editing techniques for stem cells and stem cells for in vitro study
• Presents stem cell lab design and equipment used in the lab

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Produktdetails

• Verlag: Elsevier Science & Techn.
• Seitenzahl: 240
• Erscheinungstermin: 8. Juni 2023
• Englisch
• ISBN-13: 9780128237304
• Artikelnr.: 68137155

Inhaltsangabe

1. Introduction: Stem Cell Definition, Types of Stem Cells, Use and
Application of Stem Cell
2. Extraction of Stem Cells from Animal Models
3. Harvest and Culture of Stem Cells
4. Establishment of Amniotic fluid Stem Cell
5. Stem Cell Characterization
6. Good Recording and Quality Control
7. Differentiation Techniques for Confirmation Studies
8. Transcriptomic Analysis
9. Proliferation Study and Analysis
10. Single Cell Isolation Methods and their Impact on Stem Cell Study
11. Gene Editing Techniques for Stem Cell
12. Preparation of Cells for in vitro study
13. Stem Cell Lab Design and Equipment
14. Human and Animal Research Ethics
15. Organoids