Replacing Animal Models (eBook, ePUB)

A Practical Guide to Creating and Using Culture-based Biomimetic Alternatives
Redaktion: Davies, Jamie

• Format: ePub

Produktbeschreibung

Over the last decade, in vitro models have become more sophisticated and are at a stage where they can provide an effective alternative to in vivo experiments. Replacing Animal Models provides scientists and technicians with a practical, integrated guide to developing culture-based alternatives to in vivo experiments. The book is neither political nor polemical: it is technical, illustrating by example how alternatives can be developed and used and providing useful advice on developing others. After looking at the reasons for and potential benefits of alternatives to animal experiments, the book covers a range of methods and examples emphasising the design considerations that went into each system. The chapters also include 'case studies' that illustrate the ways in which culture models can be used to answer a range of important biological questions of direct relevance to human development, physiology, disease and healing. The thesis of this book is not that all animal experimentation can be replaced, now or in the near future, by equally effective or superior alternatives. Rather, the premise is that there is substantial opportunity, here and now, to do some common types of experiment better in vitro than in vivo, and that doing so will result in both scientific and ethical gains.

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Produktdetails

• Verlag: John Wiley & Sons
• Seitenzahl: 208
• Erscheinungstermin: 19. März 2012
• Englisch
• ISBN-13: 9781119940982
• Artikelnr.: 37345449

Autorenporträt

Professor Jamie A. Davies, Centre for Integrative Physiology, Hugh Robson Building, George Square, University of Edinburgh, Edinburgh.

Inhaltsangabe

Contributors
vii Preface
xi Section 1 Introductory Material 1 Potential Advantages of Using Biomimetic Alternatives
3 Jamie Davies 2 Overview of Biomimetic Alternatives
13 Jamie Davies Section 2 Culture Methods 3 Pancreatic Islets
23 Eli C Lewis 4 Endometrial Organoid Culture
35 Merja Blauer 5 Modelling Lymphatic and Blood Capillary Patterning
45 Francoise Bruyere
Catherine Maillard
Charlotte Erpicum and Agnes Noel 6 Precision-cut Lung Slices (PCLS)
57 Christian Martin and Stefan Uhlig 7 Human Colon Tissue in Organ Culture
69 Michael K Dame and James Varani 8 Fetal Organ Culture
81 Jamie Davies 9 Design of a Mechanical Loading Device to Culture Intact Bovine Spinal Motion Segments under Multiaxial Motion
89 Jochen Walser
Stephen John Ferguson and Benjamin Gantenbein-Ritter 10 Magnetic Assembly of Tissue Surrogates
107 Chien-Yu Fu and Hwan-You Chang 11 Assembly of Renal Tissues by Cellular Self-organization
115 Mathieu Unbekandt Section 3 Case Studies of Use 12 Hierarchical Screening of Pathways: Using Cell and Organ Cultures to Reduce use of Transgenic Mice
125 Guangping Tai and Jamie Davies 13 Lung Organoid Culture to Study Responses to Viruses
137 Wenxin Wu
J Leland Booth and Jordan P Metcalf 14 Organ-cultured Human Skin for the Study of Epithelial Cell Invasion of Stroma
151 James Varani 15 Organotypic Mandibular Cultures for the Study of Inflammatory Bone Pathology
159 Alastair J Sloan
Sarah Y Taylor and Emma L Smith 16 Three-dimensional
High-density and Tissue Engineered Culture Models of Articular Cartilage
167 Ali Mobasheri
Sara Kelly
Abigail L. Clutterbuck
Constanze Buhrmann and Mehdi Shakibaei 17 Concluding Remarks
193 Jamie Davies Appendix 1 Sources of funding for development of culture-based alternatives
195 Appendix 2 Databases and web-based discussions relevant to development of alternatives
197 Index
199