Reconstituting the Cytoskeleton (eBook, ePUB)

• Format: ePub

Produktbeschreibung

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers cytoskeletal structure, including such topics as rotational movement of formins studied by fluorescence polarization microscopy, in vitro assembly assays for bacterial actin filaments, and modulators of microtubule plus end growth.

• Continues the legacy of this premier serial with quality chapters authored by leaders in the field
• Covers cytoskeletal structure
• Contains chapters with such topics as reconstitution of organelle transport and assaying microtubule nucleation by gamma-tubulin ring complex.

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Produktdetails

• Verlag: Elsevier Science & Techn.
• Seitenzahl: 512
• Erscheinungstermin: 11. März 2014
• Englisch
• ISBN-13: 9780123984807
• Artikelnr.: 41007244

Inhaltsangabe

Polymer dynamics
1. Actin filament dynamics using microfluidics
2. Bacterial actin-like proteins: purification and characterization of self-assembly properties
3. Quantitative analysis of microtubule self-assembly kinetics and tip structure

Polymer nucleation and regulation
4. Biochemical reconstitution of the WAVE regulatory complex
5. Rotational movement of formins evaluated by using single-molecule fluorescence polarization
6. Single molecule studies of actin assembly and disassembly factors
7. Assaying microtubule nucleation by the ?-tubulin ring complex
8. Reconstituting dynamic microtubule polymerization regulation by TOG domain proteins.
9. Generation of differentially modified microtubules using in vitro enzymatic approaches

Molecular motor ensembles on natural and engineered cargoes
10. Engineering defined motor ensembles with DNA origami
11. Construction and analyses of elastically-coupled multiple motor systems
12. Reconstitution of cortical dynein function
13. Reconstitution of microtubule-dependent organelle transport
14. Reconstituting the motility of isolated intracellular cargoes

Building higher order networks and interactions
15. Reconstitution of contractile actomyosin arrays
16. Directed actin assembly and motility
17. In vitro reconstitution of dynamic microtubules interacting with actin filament networks
18. Measuring kinetochore-microtubule interaction in vitro
19. Micropattern-guided assembly of overlapping pairs of dynamic microtubules

Cell extract systems
20. WAVE regulatory complex activation
21. Dissecting principles governing actin assembly using yeast extracts
23. Glycogen-supplemented mitotic cytosol for analyzing Xenopus egg microtubule organization
24. Spindle movements on immobilized chromatin micropatterns