Robert K. Scopes

Protein Purification (eBook, PDF)

Principles and Practice

• Format: PDF

Produktbeschreibung

New textbooks at all levels of chemistry appear with great regularity. Some fields such as basic biochemistry, organic re action mechanisms, and chemical thermodynamics are weil represented by many excellent texts, and new or revised editions are published sufficiently often to keep up with progress in research. However, some areas of chemistry, especially many of those taught at the graduate level, suffer from a real lack of up-to-date textbooks. The most serious needs occur in fields that are rapidly changing. Textbooks in these subjects usually have to be written by scientists actually involved in the research that is advancing the field. It is not often easy to persuade such individuals to set time aside to help spread the knowledge they have accumulated. Our goal, in this series, is to pinpoint areas of chemistry where recent progress has outpaced what is covered in any available textbooks, and then seek out and persuade experts in these fields to produce relatively concise but instruc tive introductions to their fields. These should serve the needs of one semester or one quarter graduate courses in chemistry and biochemistry. In so me cases the availability of texts in active research areas should help stimulate the creation of new courses.

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Produktdetails

• Verlag: Springer US
• Seitenzahl: 380
• Erscheinungstermin: 29. Juni 2013
• Englisch
• ISBN-13: 9781475723335
• Artikelnr.: 43987790

Inhaltsangabe

1 The Protein Purification Laboratory.
2 Making an Extract.
3 Analysis
Measurement of Protein and Enzyme Activity.
4 Separation by Precipitation.
5 Separation by Adsorption I: General Principles.
6 Separation by Adsorption II: Ion Exchangers and Nonspecific Adsorbents.
7 Separation by Adsorption
Affinity Techniques.
8 Separation in Solution.
9 Purification of Special Types of Proteins.
10 Small
Scale and Large
Scale Procedures.
11 Analysis for Purity.
12 Optimization of Procedures; Final Steps.
Appendix A.
Precipitation Tables.
Appendix B.
Solutions for Measuring Protein Concentration.
Appendix C.
Buffers for Use in Protein Chemistry.
Appendix D.
Chromatographic Materials.
References.

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1 The Protein Purification Laboratory.- 2 Making an Extract.- 3 Analysis-Measurement of Protein and Enzyme Activity.- 4 Separation by Precipitation.- 5 Separation by Adsorption I: General Principles.- 6 Separation by Adsorption II: Ion Exchangers and Nonspecific Adsorbents.- 7 Separation by Adsorption-Affinity Techniques.- 8 Separation in Solution.- 9 Purification of Special Types of Proteins.- 10 Small-Scale and Large-Scale Procedures.- 11 Analysis for Purity.- 12 Optimization of Procedures; Final Steps.- Appendix A.- Precipitation Tables.- Appendix B.- Solutions for Measuring Protein Concentration.- Appendix C.- Buffers for Use in Protein Chemistry.- Appendix D.- Chromatographic Materials.- References.

Rezensionen

From reviews of earlier editions: "good practical advice that is presented in a pleasantly readable form" (Analytical Biochemistry), "well organized and written clearly" (American Scientist), "should be on every laboratory shelf where protein are being handled or purified...a feast and a genuine pleasure to read" (Nature)