Protein Aggregation in Bacteria (eBook, PDF)

Functional and Structural Properties of Inclusion Bodies in Bacterial Cells
Redaktion: Doglia, Silvia Maria; Lotti, Marina

• Format: PDF

Produktbeschreibung

Focuses on the aggregation of recombinant proteins in bacterial cells in the form of inclusion bodies--and on their use in biotechnological and medical applications The first book devoted specifically to the topic of aggregation in bacteria, Protein Aggregation in Bacteria: Functional and Structural Properties of Inclusion Bodies in Bacterial Cells provides a large overview of protein folding and aggregation, including cell biology and methodological aspects. It summarizes, for the first time in one book, ideas and technical approaches that pave the way for a direct use of inclusion bodies in biotechnological and medical applications. Protein Aggregation in Bacteria covers: * Molecular and cellular mechanisms of protein folding, aggregation, and disaggregation in bacteria * Physiological importance and consequences of aggregation for the bacterial cell * Factors inherent to the protein sequence responsible for aggregation and evolutionary mechanisms to keep proteins soluble * Structural properties of proteins expressed as soluble aggregates and as inclusion bodies within bacterial cells both from a methodological point of view and with regard to their similarity with amyloids * Control of the structural and functional properties of aggregated proteins and use thereof in biotechnology and medicine Protein Aggregation in Bacteria is ideal for researchers in protein science, biochemistry, bioengineering, biophysics, microbiology, medicine, and biotechnology, particularly if they are related with the production of recombinant proteins and pharmaceutical science.

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Produktdetails

• Verlag: John Wiley & Sons
• Seitenzahl: 284
• Erscheinungstermin: 3. April 2014
• Englisch
• ISBN-13: 9781118855034
• Artikelnr.: 40780703

Autorenporträt

SILVIA MARIA DOGLIA, PhD, is Professor of Physics at the University of Milano-Bicocca, Italy. She received her Laurea degree in Physics at the University of Milano. She has been Staff Research Scientist of the Italian National Research Council; Visiting Scientist at the University of Stockholm; Visiting Professor at the Universities of Orléans (Fr) and of Reims (Fr). Her research in Biophysics at the University of Milano-Bicocca is focused on the study of protein folding and aggregation in vitro and in situ. MARINA LOTTI, PhD, is Professor of Biochemistry at the University of Milano-Bicocca, Italy, where she leads the group Protein Engineering and Industrial Enzymology, and is the Head of the Department of Biotechnology and Biosciences. She obtained her PhD degree at the Max-Planck Institute of Molecular Genetics, Berlin, and was a researcher of the Italian National Research Council. Major research topics include the production of recombinant proteins, protein aggregation, cold-active enzymes, and intrinsically disordered proteins.

Inhaltsangabe

Contributors vii Preface xi Introduction to the Wiley Series in Protein and
Peptide Science xiii 1 Fundamentals of Protein Folding 1 Vladimir N.
Uversky 2 Recruiting Unfolding Chaperones to Solubilize Misfolded
Recombinant Proteins 63 Rayees U.H. Mattoo and Pierre Goloubinoff 3
Osmolytes as Chemical Chaperones to Use in Protein Biotechnology 77 Ario de
Marco 4 Inclusion Bodies in the Study of Amyloid Aggregation 93 Anna
Villar-Piqué and Salvador Ventura 5 Protein Aggregation in Unicellular
Eukaryotes 117 Marina Caldara, Joris Winderickx, and Vanessa Franssens 6
Structural Properties of Bacterial Inclusion Bodies 151 Antonino Natalello,
Diletta Ami, and Silvia Maria Doglia 7 Residue-Specific Structural Studies
of Inclusion Bodies 181 Christian Wasmer, Marielle Wälti, Yongli Chen, and
Lei Wang 8 Biomedical Applications of Bacterial Inclusion Bodies 203 Imma
Ratera, Spela Peternel, Joaquin Seras-Franzoso, Olivia Cano-Garrido, Elena
García-Fruitós, Rafael Cubarsí, Esther Vazquez, José Luis Corchero,
Escarlata Rodríguez-Carmona, Jaume Veciana, and Antonio Villaverde 9
Aggregation of Recombinant Proteins: Understanding Basic Issues to Overcome
Production Bottlenecks 221 Marina Lotti and Loredano Pollegioni 10 Fusion
to a Pull-Down Module: Designing Enzymes to Form Biocatalytically Active
Insoluble Aggregates 247 Bernd Nidetzky Index 263