Andrés Illanes, Lorena Wilson, Carlos Vera

Problem Solving in Enzyme Biocatalysis (eBook, PDF)

• Format: PDF

Produktbeschreibung

Enzyme biocatalysis is a fast-growing area in process biotechnology that has expanded from the traditional fields of foods, detergents, and leather applications to more sophisticated uses in the pharmaceutical and fine-chemicals sectors and environmental management. Conventional applications of industrial enzymes are expected to grow, with major opportunities in the detergent and animal feed sectors, and new uses in biofuel production and human and animal therapy. In order to design more efficient enzyme reactors and evaluate performance properly, sound mathematical expressions must be developed which consider enzyme kinetics, material balances, and eventual mass transfer limitations. With a focus on problem solving, each chapter provides abridged coverage of the subject, followed by a number of solved problems illustrating resolution procedures and the main concepts underlying them, plus supplementary questions and answers. Based on more than 50 years of teaching experience, Problem Solving in Enzyme Biocatalysis is a unique reference for students of chemical and biochemical engineering, as well as biochemists and chemists dealing with bioprocesses. Contains: Enzyme properties and applications; enzyme kinetics; enzyme reactor design and operation 146 worked problems and solutions in enzyme biocatalysis.

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Produktdetails

• Verlag: John Wiley & Sons
• Seitenzahl: 344
• Erscheinungstermin: 2. Oktober 2013
• Englisch
• ISBN-13: 9781118341759
• Artikelnr.: 39621910

Autorenporträt

Andrés Illanes is Professor in the School of Biochemical Engineering at Pontificia Universidad Católica de Valparaíso, Chile. He has been researching enzyme biocatalysis since the 1970s, having done research in the main topics related to enzyme technology, and taught many courses at the undergraduate, M.Sc and Ph.D level in the subject both in Chile and abroad. He has authored over 80 ISI journal publications, several book chapters and three books on this topic, the latest with Springer 2008 Enzyme Biocatalysis: Principles and Applications. Lorena Wilson is Associate Professor at the School of Biochemical Engineering, Pontificia Universidad Católica de Valparaíso. She has worked on enzyme biocatalysis since her time as an undergraduate and done research in aspects related mostly to biocatalyst engineering and enzyme reactor performance. She has more than ten years teaching experience focused mostly on the subject of enzyme biocatalysis. She is a Biochemical Engineer with a PhD from the Universidad Autónoma de Madrid, Spain. Dr Wilson is also author of more than 40 ISI publications in high ranked journals and several book chapters. Carlos Vera works in the School of Biochemical Engineering at Pontificia Universidad Católica de Valparaíso, Chile.

Inhaltsangabe

Preface ix Nomenclature xi Epsilon Software Information xxi 1 Facts and
Figures in Enzyme Biocatalysis 1 1.1 Introduction 1 1.2 Enzymes as Process
Catalysts 3 1.3 Evolution of Enzyme Biocatalysis: From Hydrolysis to
Synthesis 5 1.4 The Enzyme Market: Figures and Outlook 6 References 7 2
Enzyme Kinetics in a Homogeneous System 11 2.1 Introduction 11 2.2 Theory
of Enzyme Kinetics 14 2.3 Single-Substrate Reactions 17 2.4
Multiple-Substrate Reactions 19 2.5 Multiple-Enzyme Reactions 21 2.6
Determination of Kinetic Parameters 22 2.7 Effects of Operational Variables
on Enzyme Kinetics 24 Solved Problems 29 Supplementary Problems 72
References 84 3 Enzyme Kinetics in a Heterogeneous System 87 3.1
Introduction 87 3.2 Immobilization of Enzymes 87 3.3 Mass-Transfer
Limitations in Enzyme Catalysis 92 3.4 Determination of Intrinsic Kinetic
and Mass-Transfer Parameters 102 Solved Problems 105 Supplementary Problems
127 References 138 4 Enzyme Reactor Design and Operation under Ideal
Conditions 141 4.1 Modes of Operation and Reactor Configurations 141 4.2
Definition of Ideal Conditions 142 4.3 Strategy for Reactor Design and
Performance Evaluation 143 4.4 Mathematical Models for Enzyme Kinetics,
Modes of Operation, and Reactor Configurations under Ideal Conditions 143
Solved Problems 157 Supplementary Problems 174 References 179 5 Enzyme
Reactor Design and Operation under Mass-Transfer Limitations 181 5.1
Sequential Batch and Continuously Operated Reactors with Immobilized
Enzymes 182 5.2 Mathematical Models for Enzyme Kinetics, Modes of
Operation, and Reactor Configurations under Mass-Transfer Limitations 183
Solved Problems 185 Supplementary Problems 198 6 Enzyme Reactor Design and
Operation under Biocatalyst Inactivation 203 6.1 Mechanistically Based
Mathematical Models of Enzyme Inactivation 203 6.2 Effect of Catalytic
Modulators on Enzyme Inactivation 205 6.3 Mathematical Models for Different
Enzyme Kinetics, Modes of Operation, and Reactor Configurations under
Biocatalyst Inactivation 206 6.4 Mathematical Models for Enzyme Kinetics,
Modes of Operation, and Reactor Configurations under Simultaneous
Mass-Transfer Limitations and Enzyme Inactivation 212 6.5 Strategies for
Reactor Operation under Biocatalyst Inactivation 213 Solved Problems 215
Supplementary Problems 233 References 240 7 Optimization of Enzyme Reactor
Operation 243 7.1 Strategy for the Optimization of Enzyme Reactor
Performance 244 7.2 Mathematical Programming for Static Optimization 247
7.3 Dynamic Programming 248 7.4 Statistical Optimization by Surface
Response Methodology 249 Solved Problems 254 Supplementary Problems 272
References 275 Appendix A Mathematical Methods 277 A.1. Newton's Method 277
A.2. Curve Fitting by Least Squares 280 A.3. Solving Ordinary Differential
Equations 296 A.4. Numerical Methods for Solving Differential Equations 302
References 310 Index 311