Plant Nitric Oxide (eBook, PDF)

Methods and Protocols
Redaktion: Gupta, Kapuganti Jagadis

• Format: PDF

Produktbeschreibung

With numerous methods available for the quantification of nitric oxide (NO), this detailed book explores their advantages and disadvantages in order to provide a foundation for further research in plant nitric oxide. After providing a useful practical guide to choosing a technique for measuring NO from plant materials, the book continues with chapters on chemiluminescence, diaminofluorescence (DAF), EPR spectroscopy, a laser-based method, as well as many other topics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory methods, and tips on troubleshooting and avoiding known pitfalls.

Practical and authoritative, PlantNitric Oxide: Methods and Protocols serves as a valuable guide to all researchers working and intending to work in the field of plant nitric oxide research.

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Produktdetails

• Verlag: Springer New York
• Seitenzahl: 203
• Erscheinungstermin: 19. April 2016
• Englisch
• ISBN-13: 9781493936007
• Artikelnr.: 64700702

Inhaltsangabe

An Overview of Methods in Plant Nitric Oxide (NO) Research:Why Do We Always Need to Use Multiple Methods?.- Chemiluminescence Detection ofNitric Oxide from Roots, Leaves, and Root Mitochondria.- Nitric Oxide Measurementfrom Purified Enzymes and Estimation of Scavenging Activity by Gas PhaseChemlimunimescence Method.- Localization of Nitric Oxide in Wheat Roots by DAFFluorescence.- Nitric Oxide (NO) Measurements in Stomatal Guard Cells.- Methodsto Detect Nitric Oxide in Plants: Are DAFs Really Measuring NO?.- A NovelProtocol for Detection of Nitric Oxide in Plants.- Detection of Nitric Oxide byElectron Paramagnetic Resonance Spectroscopy: Spin-Trapping withIron-Dithiocarbamates.- Measurement of Nitric Oxide (NO) Generation Rate byChloroplasts Employing Electron Spin Resonance (ESR).- Laser-Based Methods forDetection of Nitric Oxide in Plants.- Electrochemical Detection of Nitric Oxidein Plant Cell Suspensions.- Quantification and Localization of S-Nitrosothiols(SNOs) in Higher Plants.- Analysis of the Expression and Activity of NitricOxide Synthase from Marine Photosynthetic Microorganisms.- Identification ofS-Nitrosothiols by the Sequential Cysteine Blocking Technique.- Detection ofS-Nitrosoglutathione Reductase Activity in Plants.- Detection of Peroxynitritein Plants Exposed to Bacterial Infection.