Neurodegeneration Methods and Protocols (eBook, PDF)

Redaktion: Harry, Jean; Tilson, Hugh A.

• Format: PDF

Produktbeschreibung

In Neurodegeneration Methods and Protocols, Jean Harry and Hugh Tilson bring together a collection of detailed, state-of-the-art techniques for studying many aspects of nervous system cell biology. The methods span a multidisciplinary range of cellular and molecular approaches to both normal and injured nervous system function, and particularly to neurodegenerative processes. Included are basic RT-PCR techniques, cell culture systems, second-messenger signaling methods, and patch-clamp techniques. Cutting-edge and authoritative, Neurodegeneration Methods and Protocols will illuminate nervous system function, injury, degeneration, and the repair/regenerative process. These powerful techniques will also allow established laboratories-both experimental and clinical-to expand their array of methods for solving research problems and help utilize multidisciplinary procedures beyond their experience.

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Produktdetails

• Verlag: Humana Press
• Seitenzahl: 306
• Erscheinungstermin: 1. Februar 2008
• Englisch
• ISBN-13: 9781592596041
• Artikelnr.: 69699138

Inhaltsangabe

to Polymerase Chain Reaction.- Methodological Issues in Polymerase Chain Reaction for RNA.- Subtractive Hybridization of cDNA Libraries.- The Use of Differential Display RT-PCR for Identifying Altered Gene Expression in Ischemic Brain Injury.- Analysis of Gene Expression by Multiprobe RNase Protection Assay.- Molecular Probes for PNS Neurotoxicity, Degeneration, and Regeneration.- Mobility-Shift DNA-Binding Assay Using Gel Electrophoresis.- Transfection of Mammalian Cells In Vitro: Use in Analysis of Neuronal Damage.- Detection of Apoptotic Cells in the Nervous System.- Translocation Assays of Protein Kinase C Activation.- Astrocyte and Neuron Coculturing Method.- Viability Assays for Cells In Vitro: The Ethidium/Calcein Assay and the Immunofluorescence Combination Assay.- Measurement of the Nitric Oxide Synthase Activity Using the Citrulline Assay.- Measurement of Intraneuronal Free Calcium Using the Fluorescent Probe Technique.- Measurement of Calcium Buffering by Intracellular Organelles in Brain.- Receptor-Mediated Release of Inositolphosphates in Brain Slices.- The Use of Enzyme-Linked Immunosorbent Assays to Quantitate Proteins in Biological Functions.- Assays for the Analysis of Synaptic Proteins in Neurodegenerative Disorders.- Zymographic Method for the Measurement of Gelatinase Activity in Brain Tissue.- The Measurement of Monoamine Neurotransmitters in Microdialysis Perfusates Using HPLC-ECD.- Biochemical Measurement of Cholinesterase Activity.- Functional Study of Glutamate Receptor Channels in Brain Slices.- Selective Silver Staining for Neural Degeneration.- Immunohistological Markers of Cerebrovascular Integrity.- Electron Probe X-Ray Microanalysis: A Quantitative Electron Microscopy Technique for Measurement of Elements and Water in Nervous Tissue Cells.

Rezensionen

". . .the authors are to be commended for the consistency in format and altruism shown contributors to represent the procedures with minimal self-serving recounting of their use of the method."-The Quarterly Review of Biology