Monoclonal Antibodies and Functional Cell Lines (eBook, PDF)

Progress and Applications
Redaktion: Kennett, Roger H.

• Format: PDF

Produktbeschreibung

This volume serves as a follow-up to our previous book, MonoclonalAntibodies Hybridomas: A New Dimension in Biological Analyses. We continue the theme of monoclonal antibodies and their applications, attempting to cover some of the areas not covered in the previous volume. We again include an appendix de scribing methods useful to those who ar-e beginning to apply these techniques in their own laboratories. This volume will be followed by another concentrating on the combination of monoclonal antibody techniques with molecular genetic techniques to study structure/function relationships at the level of both the gene and gene product. Roger H. Kennett Kathleen B. Bechtol Philadelphia, Pennsylvania Thomas J. McKearn Princeton, New Jersey IX Acknowledgments Roger Kennett acknowledges the patience and support of his wife, Carol, and his family, friends, and colleagues during the work on this volume, and again thanks, above all, the Lord, Jesus Christ. Kathleen Bechtol wishes to thank colleagues and friends for their support and understanding during the months of preparation of this volume. Tom McKearn acknowledges and thanks his wife, Pat, and his family for their support and encouragement. Xl Contents PART I INTRODUCTION 1 Introduction: Reflections on Nine Years of Monoclonal Antibodies from Hybridomas 3 ROGER H. KENNETT, KATHLEEN B. BECHTOL, AND THOMAS J. McKEARN 1. Biotechnology'S "Coming of Age". . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 II. Monoclonal Antibodies-An Overview of Applications. . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 III. Commercialization of Monoclonal Antibody Technology.. . . .. ... . .... .... .. . ... . . 10 References. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 . . . . . . . . . . . . . . . . . . . . .

---

Dieser Download kann aus rechtlichen Gründen nur mit Rechnungsadresse in A, B, BG, CY, CZ, D, DK, EW, E, FIN, F, GR, HR, H, IRL, I, LT, L, LR, M, NL, PL, P, R, S, SLO, SK ausgeliefert werden.

Produktdetails

• Verlag: Springer New York
• Seitenzahl: 426
• Erscheinungstermin: 8. März 2013
• Englisch
• ISBN-13: 9781468446739
• Artikelnr.: 44177260

Inhaltsangabe

I Introduction.- 1 Introduction: Reflections on Nine Years of Monoclonal Antibodies from Hybridomas.- II Characterization of the Detailed Antigenic Structure of Macromolecules.- 2 Monoclonal Antibodies Directed against the Nicotinic Acetylcholine Receptor.- 3 Monoclonal Antibodies to Enzymes.- 4 Monoclonal Antibodies Directed to Cell-Surface Carbohydrates.- III Detection and Isolation of Antigens in Complex Biological Systems.- 5 Adhesion-Related Integral Membrane Glycoproteins Identified by Monoclonal Antibodies.- 6 Monoclonal Antibodies to Cytoskeletal Proteins.- 7 Molecular Bases of Neuronal Individuality: Lessons from Anatomical and Biochemical Studies with Monoclonal Antibodies.- IV Toward Practical Applications in Medicine.- 8 Monoclonal Antibodies to Human Lymphocytes: Clinical Application in the Therapy of Leukemia.- 9 Monoclonal Antibodies to Human Neuroblastoma Cells and Other Solid Tumors.- 10 Monoclonal Antibodies and Immunoparasitology.- V Developing Areas of Biotechnology.- 11 Production of Human Monoclonal Antibodies.- 12 Monoclonal Antibodies and Molecular Genetics: Oncogenes and Oncogene Products.- 13 Functional Murine T-Cell Clones.- Appendix Additional Methods for Production and Characterization of Monoclonal Antibodies and Continuous Cell Lines.- Zdenka L. Jonak and Roger H. Kennett.- Fusion of Nonadherent Human Cell Lines.- Kenneth E. Truitt, James W. Larrick, and James W. Larrick.- ELISA for Immunoglobulin Subclass Determination.- Anselmo Otero and Roger H. Kennett.- Immunofiltration: A Rapid Screening Assay for Detection of Antibodies Directed against Cell Surface Antigens.- Elliott K. Main, Mary Kate Hart, and Darcy B. Wilson.- Binding Inhibition by Cell Extracts.- Lois Alterman Lampson.- Immunoblots and Autoradiography Used to Analyze the Proteins Recognized by Monoclonal Antibodies.- Cheryl A. Fisher and Lois Alterman Lampson.- Peroxidase as an Alternative to Radiolabels for Immunoblotting Assays.- Gerd G. Maul.- The Use of Streptavidin in the Detection of Monoclonal Antibodies.- Rosemary J. Versteegen and Christine Clark.- Solid Phase Identification and Molecular Weight Determination of Cell Membrane Antigens with Monoclonal Antibodies.- Roberto L. Ceriani.- Antigen Localization in Tissue Sections.- Kathleen B. Bechtol.- Microscopic Analysis of Monoclonal Antibody Binding to Agarose-Embedded Cell Lines.- Lois Alterman Lampson, James P. Whelan, and Carol J. Lawton.- Derivation of T-Cell Clones.- Andrew L. Glasebrook and Frank W. Fitch.- Methods for Transfection of Human DNA into Primary Mouse Lymphocytes and NIH/3T3 Mouse Fibroblasts.- Zdenka L. Jonak and Roger H. Kennett.- Cell Lines for Hybridoma Formation and References to Other Methods.