Molecular Fluorescence (eBook, PDF)
Principles and Applications
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Molecular Fluorescence (eBook, PDF)
Principles and Applications
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This second edition of the well-established bestseller is completely updated and revised with approximately 30 % additional material, including two new chapters on applications, which has seen the most signifi cant developments. The comprehensive overview written at an introductory level covers fundamental aspects, principles of instrumentation and practical applications, while providing many valuable tips. For photochemists and photophysicists, physical chemists, molecular physicists, biophysicists, biochemists and biologists, lecturers and students of chemistry, physics, and biology.
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- Produktdetails
- Verlag: Wiley-VCH GmbH
- Seitenzahl: 570
- Erscheinungstermin: 27. März 2013
- Englisch
- ISBN-13: 9783527650033
- Artikelnr.: 38485983
- Verlag: Wiley-VCH GmbH
- Seitenzahl: 570
- Erscheinungstermin: 27. März 2013
- Englisch
- ISBN-13: 9783527650033
- Artikelnr.: 38485983
Phosphorescence Photoluminescence of Organic and Inorganic Species:
Fluorescence or Phosphorescence? Various De-Excitation Processes of Excited
Molecules Fluorescent Probes, Indicators, Labels, and Tracers Ultimate
Temporal and Spatial Resolution: Femtoseconds, Femtoliters, Femtomoles, and
Single-Molecule Detection PART I: PRINCIPLES ABSORPTION OF ULTRAVIOLET,
VISIBLE, AND NEAR-INFRARED RADIATION Electronic Transitions Transition
Probabilities: The Beer - Lambert Law, Oscillator Strength Selection Rules
The Franck - Condon Principle Multiphoton Absorption and Harmonic
Generation CHARACTERISTICS OF FLUORESCENCE EMISSION Radiative and
Nonradiative Transitions between Electronic States Lifetimes and Quantum
Yields Emission and Excitation Spectra STRUCTURAL EFFECTS ON FLUORESCENCE
EMISSION Effects of the Molecular Structure of Organic Molecules on Their
Fluorescence Fluorescence of Conjugated Polymers (CPs) Luminescence of
Carbon Nanostructures: Fullerenes, Nanotubes, and Carbon Dots Luminescence
of Metal Compounds, Metal Complexes, and Metal Clusters Luminescence of
Semiconductor Nanocrystals (Quantum Dots and Quantum Rods) ENVIRONMENTAL
EFFECTS ON FLUORESCENCE EMISSION Homogeneous and Inhomogeneous Band
Broadening - Red-Edge Effects General Considerations on Solvent Effects
Solvent Relaxation Subsequent to Photoinduced Charge Transfer (PCT) Theory
of Solvatochromic Shifts Effects of Specific Interactions Empirical Scales
of Solvent Polarity Viscosity Effects Fluorescence in Gas Phase: Supersonic
Jets EFFECTS OF INTERMOLECULAR PHOTOPHYSICAL PROCESSES ON FLUORESCENCE
EMISSION Introduction Overview of the Intermolecular De-Excitation
Processes of Excited Molecules Leading to Fluorescence Quenching
Photoinduced Electron Transfer Formation of Excimers and Exciplexes
Photoinduced Proton Transfer FLUORESCENCE POLARIZATION: EMISSION ANISOTROPY
Polarized Light and Photoselection of Absorbing Molecules Characterization
of the Polarization State of Fluorescence (Polarization Ratio and Emission
Anisotropy) Instantaneous and Steady-State Anisotropy Additivity Law of
Anisotropy Relation between Emission Anisotropy and Angular Distribution of
the Emission Transition Moments Case of Motionless Molecules with Random
Orientation Effect of Rotational Motion Applications EXCITATION ENERGY
TRANSFER Introduction Distinction between Radiative and Nonradiative
Transfer Radiative Energy Transfer Nonradiative Energy Transfer
Determination of Distances at a Supramolecular Level Using FRET FRET in
Ensembles of Donors and Acceptors FRET between Like Molecules: Excitation
Energy Migration in Assemblies of Chromophores Overview of Qualitative and
Quantitative Applications of FRET PART II: TECHNIQUES STEADY-STATE
SPECTROFL UOROMETRY Operating Principles of a Spectrofl uorometer
Correction of Excitation Spectra Correction of Emission Spectra Measurement
of Fluorescence Quantum Yields Possible Artifacts in Spectrofl uorometry
Measurement of Steady-State Emission Anisotropy: Polarization Spectra
TIME-RESOLVED FLUORESCENCE TECHNIQUES Basic Equations of Pulse and
Phase-Modulation Fluorimetries Pulse Fluorimetry Phase-Modulation
Fluorimetry Artifacts in Time-Resolved Fluorimetry Data Analysis Lifetime
Standards Time-Resolved Polarization Measurements Time-Resolved
Fluorescence Spectra Lifetime-Based Decomposition of Spectra Comparison
between Single-Photon Timing Fluorimetry and Phase-Modulation Fluorimetry
FLUORESCENCE MICROSCOPY Wide-Field (Conventional), Confocal, and Two-Photon
Fluorescence Microscopies Super-Resolution (Subdiffraction) Techniques
Fluorescence Lifetime Imaging Microscopy (FLIM) Applications FLUORESCENCE
CORRELATION SPECTROSCOPY AND SINGLE-MOLECULE FLUORESCENCE SPECTROSCOPY
Fluorescence Correlation Spectroscopy (FCS) Single-Molecule Fluorescence
Spectroscopy PART III: APPLICATIONS EVALUATION OF LOCAL PHYSICAL PARAMETERS
BY MEANS OF FLUORESCENT PROBES Fluorescent Probes for Polarity Estimation
of 'Microviscosity', Fluidity, and Molecular Mobility Temperature Pressure
CHEMICAL SENSING VIA FLUORESCENCE Introduction Various Approaches of
Fluorescence Sensing Fluorescent pH Indicators 412 Transfer (PET) Design
Principles of Fluorescent Molecular Sensors Based on Ion or Molecule
Recognition Fluorescent Molecular Sensors of Metal Ions Fluorescent
Molecular Sensors of Anions Fluorescent Molecular Sensors of Neutral
Molecules Fluorescence Sensing of Gases Sensing Devices Remote Sensing by
Fluorescence LIDAR AUTOFL UORESCENCE AND FLUORESCENCE LABELING IN BIOLOGY
AND MEDICINE Introduction Natural (Intrinsic) Chromophores and Fluorophores
Fluorescent Proteins (FPs) Fluorescent Small Molecules Quantum Dots and
Other Luminescent Nanoparticles Conclusion MISCELLANEOUS APPLICATIONS
Fluorescent Whitening Agents Fluorescent Nondestructive Testing Food
Science Forensics Counterfeit Detection Fluorescence in Art APPENDIX:
CHARACTERISTICS OF FLUORESCENT ORGANIC COMPOUNDS INDEX
Phosphorescence Photoluminescence of Organic and Inorganic Species:
Fluorescence or Phosphorescence? Various De-Excitation Processes of Excited
Molecules Fluorescent Probes, Indicators, Labels, and Tracers Ultimate
Temporal and Spatial Resolution: Femtoseconds, Femtoliters, Femtomoles, and
Single-Molecule Detection PART I: PRINCIPLES ABSORPTION OF ULTRAVIOLET,
VISIBLE, AND NEAR-INFRARED RADIATION Electronic Transitions Transition
Probabilities: The Beer - Lambert Law, Oscillator Strength Selection Rules
The Franck - Condon Principle Multiphoton Absorption and Harmonic
Generation CHARACTERISTICS OF FLUORESCENCE EMISSION Radiative and
Nonradiative Transitions between Electronic States Lifetimes and Quantum
Yields Emission and Excitation Spectra STRUCTURAL EFFECTS ON FLUORESCENCE
EMISSION Effects of the Molecular Structure of Organic Molecules on Their
Fluorescence Fluorescence of Conjugated Polymers (CPs) Luminescence of
Carbon Nanostructures: Fullerenes, Nanotubes, and Carbon Dots Luminescence
of Metal Compounds, Metal Complexes, and Metal Clusters Luminescence of
Semiconductor Nanocrystals (Quantum Dots and Quantum Rods) ENVIRONMENTAL
EFFECTS ON FLUORESCENCE EMISSION Homogeneous and Inhomogeneous Band
Broadening - Red-Edge Effects General Considerations on Solvent Effects
Solvent Relaxation Subsequent to Photoinduced Charge Transfer (PCT) Theory
of Solvatochromic Shifts Effects of Specific Interactions Empirical Scales
of Solvent Polarity Viscosity Effects Fluorescence in Gas Phase: Supersonic
Jets EFFECTS OF INTERMOLECULAR PHOTOPHYSICAL PROCESSES ON FLUORESCENCE
EMISSION Introduction Overview of the Intermolecular De-Excitation
Processes of Excited Molecules Leading to Fluorescence Quenching
Photoinduced Electron Transfer Formation of Excimers and Exciplexes
Photoinduced Proton Transfer FLUORESCENCE POLARIZATION: EMISSION ANISOTROPY
Polarized Light and Photoselection of Absorbing Molecules Characterization
of the Polarization State of Fluorescence (Polarization Ratio and Emission
Anisotropy) Instantaneous and Steady-State Anisotropy Additivity Law of
Anisotropy Relation between Emission Anisotropy and Angular Distribution of
the Emission Transition Moments Case of Motionless Molecules with Random
Orientation Effect of Rotational Motion Applications EXCITATION ENERGY
TRANSFER Introduction Distinction between Radiative and Nonradiative
Transfer Radiative Energy Transfer Nonradiative Energy Transfer
Determination of Distances at a Supramolecular Level Using FRET FRET in
Ensembles of Donors and Acceptors FRET between Like Molecules: Excitation
Energy Migration in Assemblies of Chromophores Overview of Qualitative and
Quantitative Applications of FRET PART II: TECHNIQUES STEADY-STATE
SPECTROFL UOROMETRY Operating Principles of a Spectrofl uorometer
Correction of Excitation Spectra Correction of Emission Spectra Measurement
of Fluorescence Quantum Yields Possible Artifacts in Spectrofl uorometry
Measurement of Steady-State Emission Anisotropy: Polarization Spectra
TIME-RESOLVED FLUORESCENCE TECHNIQUES Basic Equations of Pulse and
Phase-Modulation Fluorimetries Pulse Fluorimetry Phase-Modulation
Fluorimetry Artifacts in Time-Resolved Fluorimetry Data Analysis Lifetime
Standards Time-Resolved Polarization Measurements Time-Resolved
Fluorescence Spectra Lifetime-Based Decomposition of Spectra Comparison
between Single-Photon Timing Fluorimetry and Phase-Modulation Fluorimetry
FLUORESCENCE MICROSCOPY Wide-Field (Conventional), Confocal, and Two-Photon
Fluorescence Microscopies Super-Resolution (Subdiffraction) Techniques
Fluorescence Lifetime Imaging Microscopy (FLIM) Applications FLUORESCENCE
CORRELATION SPECTROSCOPY AND SINGLE-MOLECULE FLUORESCENCE SPECTROSCOPY
Fluorescence Correlation Spectroscopy (FCS) Single-Molecule Fluorescence
Spectroscopy PART III: APPLICATIONS EVALUATION OF LOCAL PHYSICAL PARAMETERS
BY MEANS OF FLUORESCENT PROBES Fluorescent Probes for Polarity Estimation
of 'Microviscosity', Fluidity, and Molecular Mobility Temperature Pressure
CHEMICAL SENSING VIA FLUORESCENCE Introduction Various Approaches of
Fluorescence Sensing Fluorescent pH Indicators 412 Transfer (PET) Design
Principles of Fluorescent Molecular Sensors Based on Ion or Molecule
Recognition Fluorescent Molecular Sensors of Metal Ions Fluorescent
Molecular Sensors of Anions Fluorescent Molecular Sensors of Neutral
Molecules Fluorescence Sensing of Gases Sensing Devices Remote Sensing by
Fluorescence LIDAR AUTOFL UORESCENCE AND FLUORESCENCE LABELING IN BIOLOGY
AND MEDICINE Introduction Natural (Intrinsic) Chromophores and Fluorophores
Fluorescent Proteins (FPs) Fluorescent Small Molecules Quantum Dots and
Other Luminescent Nanoparticles Conclusion MISCELLANEOUS APPLICATIONS
Fluorescent Whitening Agents Fluorescent Nondestructive Testing Food
Science Forensics Counterfeit Detection Fluorescence in Art APPENDIX:
CHARACTERISTICS OF FLUORESCENT ORGANIC COMPOUNDS INDEX
- Angewandte Chemie (international edition), 2002; Vol. 41 No. 16