Molecular Diagnosis of Infectious Diseases (eBook, PDF)

Redaktion: Decker, Jochen; Reischl, Udo

• Format: PDF

Produktbeschreibung

In this second edition of a much-acclaimed classic laboratory manual, Molecular Diagnosis of Infectious Diseases, leading experts from academia and industry describe cutting-edge methods for the protein-based diagnosis of infectious diseases. Explaining the latest developments in genomics, proteomics, bioinformatics, biosensors, high-throughput devices, and recombinant technology, the authors demonstrate the power of these new methodologies in both established and novel procedures by applying them successfully to the identification and characterization of valuable diagnostic markers, immunomodulatory components, epitope mapping, the production and purification of recombinant antigens, and their use in diagnostic reagents in immunological assays. Basic problems of serological laboratory diagnostics and the use of immunoproteomics for discovering target proteins are also addressed. Each readily reproducible protocol is described in step-by-step detail and includes a background introduction, equipment and reagent lists, tips on troubleshooting and avoiding known pitfalls, and extensive references. Comprehensive and up-to-date, Molecular Diagnosis of Infectious Diseases, Second Edition offers novice investigators an easy entry into the field and experienced researchers a broad array of time saving tools that will not only elucidate new principles, but also greatly facilitate the development and successful application of rapid diagnostic methods.

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Produktdetails

• Verlag: Humana Press
• Seitenzahl: 464
• Erscheinungstermin: 1. Februar 2008
• Englisch
• ISBN-13: 9781592596799
• Artikelnr.: 69699170

Inhaltsangabe

Protein-Based Identification Of Antigens.- Proteomic Approaches to Antigen Discovery.- Immunoproteomics.- Immunoprecipitation and Blotting.- DNA-Based Identification of Antigens.- Representational Difference Analysis of cDNA.- Microarray Data Analysis and Mining.- Expression Cloning.- Identification of Epitopes and Immunomodulatory Components.- Determination of Epitopes by Mass Spectrometry.- Identification of T-Cell Epitopes Using ELISpot and Peptide Pool Arrays.- Virus-like Particles.- Application of Single-Cell Cultures of Mouse Splenocytes as an Assay System to Analyze the Immunomodulatory Properties of Bacterial Components.- Expression of Recombinant Proteins.- High-Throughput Expression and Purification of 6xHis-Tagged Proteins in a 96-Well Format.- Production of Antigens in Chlamydomonas reinhardtii.- Codon-Optimized Genes that Enable Increased Heterologous Expression in Mammalian Cells and Elicit Efficient Immune Responses in Mice after Vaccination of Naked DNA.- Purification, Modification, and Renaturation of Recombinant Proteins.- Purification and Immunological Characterization of Recombinant Antigens Expressed in the Form of Insoluble Aggregates (Inclusion Bodies).- Purification of Recombinant Proteins with High Isoelectric Points.- Refolding of Inclusion Body Proteins.- Small-Molecule-Protein Conjugation Procedures.- Characterization of Recombinant Proteins.- Structural Characterization of Proteins and Peptides.- Determination of Kinetic Data Using Surface Plasmon Resonance Biosensors.- Affinity Measurements of Biological Molecules by a Quartz Crystal Microbalance (QCM) Biosensor.- Evaluation of Recombinant Proteins in Immunological Test Systems.- Solid Supports in Enzyme-Linked Immunosorbent Assay and Other Solid-Phase Immunoassays.- Design and Preparationof Recombinant Antigens as Diagnostic Reagents in Solid-Phase Immunosorbent Assays.- Basic Problems of Serological Laboratory Diagnosis.- Molecular Diagnostics Resources on the Internet.- Recombinant Receptor Molecules.- Cloning Single-Chain Antibody Fragments (scFv) from Hybridoma Cells.

Rezensionen

"The comprehensive nature of the book makes it an invaluable tool for industry and academia for managing the large amount of data generated in bioinformatics. Each chapter is written in the usual scientific paper format of an abstract, introduction, materials, methods, notes or conclusions and references. The index is well done and makes the book particularly helpful as a reference manual. "-Doody's Health Sciences Book Review Journal

"...Anyone who has the interest and resources to develop novel assays not only for infectious diseases but also for other diseases will benefit tremendously from this book."
-Archives of Pathology and Laboratory Medicine

"...a valuable methodological book for molecular biologists in a hospital laboratory or in academia." -Trends in Parasitology

"...a powerful tool for developing better experiments in the diagnostics of infectious diseases." -International Microbiology

"...a very good compilation of techniques that can be used for numerous applications."
-Analytical Biochemistry

"...a useful reference source for anyone who wants to manipulate microbial protein for diagnostic purposes."
-International Journal of Medical Microbiology

"...this book continues the well-established format of this series from Humana Press in providing clearly described laboratory protocols with useful background discussion...recommended as a useful laboratory reference book..." - Proteomics

"The comprehensive nature of the book makes it an invaluable tool for industry and academia for managing the large amount of data generated in bioinformatics." -Doody's Health Sciences and Book Review Journal

"...well-organized...provides detailed methods andpractical tips...the book can be recommended for students, graduate students, and postdoctoral, fellows in the field of proteomics..." - Clinical Infectious Diseases

"...brings together the key elements of oral medicine and nutrition in an accessible form for the dental professional...an excellent reference text...wholeheartedly recommend(ed)." - American Society for Clinical Nutrition

Review of the first edition:
"This work represents one of the most up-to-date molecular biology protocols books to date. . .Anyone who has the interest and resources to develop novel assays not only for infectious diseases but also for other diseases will benefit tremendously from this book. . a valuable purchase to keep as a quick protocol source." -Archives of Pathology and Laboratory Medicine