Modifications and Targeting of Protein Termini Part A (eBook, ePUB)

• Format: ePub

Produktbeschreibung

Modifications and Targeting of Protein Termini, Volume 684 in the Methods in Enzymology series serial highlights new advances in the field, with this new volume presenting interesting chapters pn a variety of timely topics, including Optimizing purification and activity assays of N-terminal methyltransferase complexes, In vitro reconstitution of hierarchical steps of Arg/N-degron pathway, Identification of N-degrons and N-recognins by using peptide-pull-downs combined with quantitative proteomics, A decoupled Virotrap approach to study the interactomes of N-terminal proteoforms, Monitoring ADO-dependent proteolysis in cells using fluorescent reporter proteins, Site-specific a-N-terminal methylation on peptides through chemical and enzymatic methods, and more.

Additional sections cover Monitoring the interactions between N-degrons and N-recognins of the Arg/N-degron pathway, Characterization and chemical modulation of p62/SQSTM1 as an autophagic N-recognin of the Arg/N-degron pathway, Analysis of higher plant N-degron pathway components and substrates via expression in S. cerevisiae, and so much more.

• Provides the authority and expertise of leading contributors from an international board of authors
• Presents the latest release in the Methods in Enzymology serials
• Updated release includes the latest information on Modifications and Targeting of Protein Termini

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Produktdetails

• Verlag: Elsevier Science & Techn.
• Seitenzahl: 338
• Erscheinungstermin: 1. Juli 2023
• Englisch
• ISBN-13: 9780443157738
• Artikelnr.: 68118558

Inhaltsangabe

1. Selective ribosome profiling as a tool to study interactions of
translating ribosomes in mammalian cells Bernd Bukau, Jiri Koubek, Manuel
Günnigmann and Günter Kramer 2. Deformylation of nascent chains on the
ribosome Marina V. Rodnina, Lena Bögeholz, Evan Mercier and Wolfgang
Wintermeyer 3. Optimizing purification and activity assays of N-terminal
methyltransferase complexes Christine Schaner Tooley, Haley Parker and John
Tooley 4. Site-specific a-N-terminal methylation on peptides through
chemical and enzymatic methods Rong Huang and Ying Meng 5. Biochemical and
structural analysis of N-myristoyltransferase-mediated protein tagging
Thierry Meinnel, Carmela Giglione, Paul Monassa, Frédéric Rivière, Cyril
Dian and Frédéric Frottin 6. Kinetic and catalytic features of
N-myristoyltransferases Thierry Meinnel and Carmela Giglione 7. Detecting
N-terminal myristoylation using metabolic Alk12 labeling Hening Lin and
Yilai Xu 8. Peptide CoA conjugates for in situ proteomics profiling of
acetyltransferase activities Iris Finkemeier, Jürgen Eirich, Julia
Sindlinger, Stefan Schön and Dirk Schwarzer 9. A decoupled Virotrap
approach to study the interactomes of N-terminal proteoforms Kris Gevaert,
Annelies Bogaert, Tessa Van de Steene, Marnik Vuylsteke and Sven Eyckerman
10. The amino-dipeptidyl peptidases DPP8 and DPP9: purification,
interaction studies and enzymatic assays Ruth Geiss-Friedlander, Laura
Donzelli and Oguz Bolgi