Philip D. Whitfield, David Eckersall

Methods in Animal Proteomics (eBook, ePUB)

• Format: ePub

Produktbeschreibung

The study of proteomics provides researchers with a better understanding of disease and physiological processes in animals. Methods in Animal Proteomics will provide animal scientists and veterinarians currently researching these topics in domestic animals a firm foundation in the basics of proteomics methodology, while also reviewing important advances that will be of interest to established researchers in the field. Chapters will provide practical information on a range of topics including protein identification and separation, bioinformatics, and applications to disease and reproduction research. This text will be written by leading international proteomics experts and essential for researchers in the fields of animal biology and veterinary medicine.

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Produktdetails

• Verlag: John Wiley & Sons
• Seitenzahl: 464
• Erscheinungstermin: 5. Juli 2011
• Englisch
• ISBN-13: 9780470960646
• Artikelnr.: 37640913

Autorenporträt

Philip D. Whitfield is Head of Lipodomic Research at the University of the Highlands and Islands Department of Diabetes and Cardiovascular Science David Eckersall is Professor of Veterinary Biochemistry University of Glasgow.

Inhaltsangabe

SECTION I: EXPLORING ANIMAL PROTEOMES. 1. General Introduction. The basis
of proteomics to provide additional perspectives in animal biology. This
chapter will also set proteomics within the context of animal genome
programmes and provide an outline of different proteomic strategies.. 2.
Types of Samples and Experimental Planning. The types of samples used in
animal proteomics experiments and the methodologies routinely used in the
preparation of samples from the main body fluids and tissues of interest
(blood, blood cells, muscle, liver, kidney, lung, intestine. CNS, CSF,
urine, milk, venom). The analysis of proteins from different cellular
fractions will be discussed.. 3. Protein Separation Strategies. This will
cover electrophoretic and chromatographic techniques used for protein
separation and isolation such as 1D and 2D SDS-PAGE, liquid chromatography,
ion exchange chromatography and size exclusion chromatography.. 4. Methods
for Protein Identification. This chapter will discuss the mass
spectrometric analyses in protein identification. This will cover the
analyses intact proteins, the use of peptide mass fingerprinting and de
novo sequencing strategies. The variety of instrument platforms routinely
used in proteomics experiments will be also described, for example
MALDI-TOF-MS, LC-MS/MS and MudPIT. The use of antibody-based approaches
such as ELISA, protein arrays and western blotting to confirm protein
identities will be explored.. 5. Comparative Proteomic Approaches. This
chapter will discuss the theoretical and practical aspects of methods used
in comparative proteomic analysis. This will cover gel-based strategies
including the densitometric analysis of 1D and 2D gels and DIGE and the use
of mass spectrometric approaches to determine the relative and absolute
concentrations of proteins. The evaluation and interpretation of the
quantitative proteomic data and the use of univariate and multivariate
statistical analysis will also be discussed.. 6. Advancing Technologies for
Spatial and Temporal Proteomics. This chapter will outline a number of
emerging technologies that have been developed for proteome simplification,
protein turnover, post-translational modifications and protein-protein
interactions. The ability of these approaches to explore animal proteomes
greater depth will be described and how they can provide functional
information to reveal how the whole organism works.. 7. Bioinformatics and
Data Mining in Animal Proteomics. The section will outline the application
of bioinformatics in relating proteome data to animal genomes and how this
information can be used to establish testable hypotheses to address
questions of relevance to animal physiology and disease. The databases used
in the identification of proteins and the value of cross-species matching
in animal proteomics investigations will be discussed.. SECTION II:
APPLICATIONS OF PROTEOMICS IN ANIMAL BIOLOGY. 8. Application of Proteomics
to Studies on Phylogeny and Evolution. This chapter will outline the role
proteomic strategies in molecular evolution studies to the application of
these approaches to discover phylogenetic relationships among species and
the evolutionary processes that caused the sequences to divergence.. 9.
Proteomic Strategies to Investigate Adaptive Processes. Living organisms
rarely exist in unchanging environments and many external factors can
dominate their life strategies. This chapter will discuss how defining an
animal's response to changing environmental conditions at the protein level
may provide fundamental insights into the molecular basis of adaptive
processes.. 10. Proteomics in Animal Health and Disease. Studies where
proteomics has been employed to identify diagnostic biomarkers and explore
the pathophysiology of important animal diseases will be discussed. The
potential use of proteomics in clinical veterinary environments will also
be outlined.. 11. Host-Pathogen Interactions. Animals are constantly under
challenge by pathogens that have profound effects on the cells which they
invade. Understanding this interaction is of vital importance for
determining the host response to infection and for the development of novel
strategies for therapeutic intervention. This chapter will cover the
proteomic analysis of infectious agents and studies of microbial,
parasitic, viral and zoonotic infections and the use of these strategies in
the identification of vaccine candidates.. 12. Proteomics in Animal
Reproduction and Breeding. This chapter will cover the application of
proteomic approaches to investigate fertility development and reproductive
performance of production animals.. 13. Investigation of Animal Venoms and
Toxins. Animal venoms often comprise of complex mixtures of proteins. This
chapter will describe the use of proteomic technologies in characterizing
these proteins. The application of proteomic approaches to investigate the
biological and pathological activities of these venoms and to guide the
development of appropriate treatments will also be discussed.. 14.
Assessment in the Safety and Quality of Food of Animal Origin. Tracking
quality changes in food such as meat, fish, milk and cheese using
proteomics. Postmortem effects on quality and processability. Proteomics
and species authentication. The identification and characterization of
allergens.. SECTION III: CONCLUSIONS. 15. Challenges and Future Directions
for Animal Proteomics. How future developments in proteomics will lead to
improvements in the study of animal health and disease.