Stepan Podzimek

Light Scattering, Size Exclusion Chromatography and Asymmetric Flow Field Flow Fractionation (eBook, ePUB)

Powerful Tools for the Characterization of Polymers, Proteins and Nanoparticles

• Format: ePub

Produktbeschreibung

A comprehensive, practical approach to three powerful methods of polymer analysis and characterization This book serves as a complete compendium of three important methods widely used for the characterization of synthetic and natural polymers--light scattering, size exclusion chromatography (SEC), and asymmetric flow field flow fractionation (A4F). Featuring numerous up-to-date examples of experimental results obtained by light scattering, SEC, and A4F measurements, Light Scattering, Size Exclusion Chromatography and Asymmetric Flow Field Flow Fractionation takes an all-in-one approach to deliver a complete and thorough explanation of the principles, theories, and instrumentation needed to characterize polymers from the viewpoint of their molar mass distribution, size, branching, and aggregation. This comprehensive resource: * Is the only book gathering light scattering, size exclusion chromatography, and asymmetric flow field flow fractionation into a single text * Systematically compares results of size exclusion chromatography with results of asymmetric flow field flow fractionation, and how these two methods complement each other * Provides in-depth guidelines for reproducible and correct determination of molar mass and molecular size of polymers using SEC or A4F coupled with a multi-angle light scattering detector * Offers a detailed overview of the methodology, detection, and characterization of polymer branching Light Scattering, Size Exclusion Chromatography and Asymmetric Flow Field Flow Fractionation should be of great interest to all those engaged in the polymer analysis and characterization in industrial and university research, as well as in manufacturing quality control laboratories. Both beginners and experienced can confidently rely on this volume to confirm their own understanding or to help interpret their results.

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Produktdetails

• Verlag: John Wiley & Sons
• Seitenzahl: 372
• Erscheinungstermin: 20. April 2011
• Englisch
• ISBN-13: 9781118102725
• Artikelnr.: 37361755

Autorenporträt

Stepan Podzimek, an author or coauthor of forty scientific papers, works as a head of the Department of Analytical and Physical Chemistry at SYNPO, a Czech R&D company, and as a professor at the Institute of Chemistry and Technology of Macromolecular Materials at the University of Pardubice. He has been working in the field of polymer analysis and characterization for twenty-five years. His research interests include characterization of molecular and chemical structure of synthetic and natural polymers using size exclusion chromatography, liquid chromatography, asymmetric flow field flow fractionation, light scattering, viscometry, mass spectroscopy, and study of structure-property relationships. In particular, he has focused on the characterization of branched polymers.

Inhaltsangabe

Preface. 1 Polymers. 1.1 Introduction. 1.2 Molecular Structure of Polymers.
1.2.1 Macromolecules in Dilute Solution. 1.3 Molar Mass Distribution. 1.3.1
Description of Molar Mass Distribution. 1.3.1.1 Distribution Functions.
1.3.1.2 Molar Mass Averages. 1.4 Methods for the Determination of Molar
Mass. 1.4.1 Method of End Groups. 1.4.2 Osmometry. 1.4.2.1 Vapor Pressure
Osmometry. 1.4.2.2 Membrane osmometry. 1.4.3 Dilute Solution Viscometry.
1.4.3.1 Properties of Mark-Houwink Exponent. 1.4.3.2 Molecular Size from
Intrinsic Viscosity. 1.4.3.3 Dependence of Intrinsic Viscosity on Polymer
Structure, Temperature and Solvent. 1.4.4 Matrix Assisted Laser Desorption
Ionization Time-of-Flight Mass Spectrometry. 1.4.5 Analytical
Ultracentrifugation. 1.5 Keynotes. 1.6 References. 2 Light Scattering. 2.1
Theory and Basic Principles. 2.2 Types of Light Scattering. 2.2.1 Static
Light Scattering. 2.2.1.1 Particle Scattering Functions. 2.2.1.2 Light
Scattering Formalisms. 2.2.1.3 Processing the Experimental Data. 2.2.2
Dynamic Light Scattering. 2.3 Light Scattering Instrumentation. 2.4
Specific Refractive Index Increment. 2.5 Light Scattering in Batch and
Chromatography Mode. 2.6 Parameters Affecting Accuracy of Molar Mass
Determined by Light Scattering. 2.7 Examples of Light Scattering
Measurement in Batch Mode. 2.8 Keynotes. 2.9 References. 3 Size Exclusion
Chromatography. 3.1 Introduction. 3.2 Separation Mechanisms. 3.2.1 Steric
Exclusion. 3.2.2 Restricted Diffusion. 3.2.3 Separation by Flow. 3.2.4 Peak
Broadening and Separation Efficiency. 3.2.5 Secondary Separation
Mechanisms. 3.3 Instrumentation. 3.3.1 Solvents. 3.3.2 Columns and Column
Packing. 3.3.3 Detectors. 3.3.3.1 UV Detector. 3.3.3.2 Refractive Index
Detector. 3.3.3.3 Infrared Detector. 3.3.3.4 Evaporative Light Scattering
Detector. 3.3.3.5 Viscosity Detector. 3.3.3.6 Light Scattering Detector.
3.3.3.7 Other Types of Detectors. 3.4 Column Calibration. 3.4.1 Universal
Calibration. 3.4.2 Flow Marker. 3.5 SEC Measurements and Data Processing.
3.5.1 Sample Preparation. 3.5.1.1 Sample Derivatization. 3.5.2
Determination of Molar Mass and Molar Mass Distribution. 3.5.3 Reporting
Results. 3.5.4 Characterization of Chemical Composition of Copolymers and
Polymer Blends. 3.5.5 Characterization of Oligomers. 3.5.6 Influence of
Separation Conditions. 3.5.7 Accuracy, Repeatability and Reproducibility of
SEC Measurements. 3.6 Applications of SEC. 3.7 Keynotes. 3.8 References. 4
Combination of SEC and Light Scattering. 4.1 Introduction. 4.2 Data
Collection and Processing. 4.2.1 Processing MALS Data. 4.2.1.1 Debye Fit
Method. 4.2.1.2 Zimm Fit Method. 4.2.1.3 Berry fit Method. 4.2.1.4 Random
Coil Fit Method. 4.2.1.5 Influence of Light Scattering Formalism on Molar
Mass and RMS Radius. 4.2.2 Determination of Molar Mass and RMS Radius
Averages and Distributions. 4.2.3 Chromatogram Processing. 4.2.4 Influence
of Concentration and Second Virial Coefficient. 4.2.5 Repeatability and
Reproducibility. 4.2.6 Accuracy of Results. 4.3 Applications of SEC-MALS.
4.3.1 Determination of Molar Mass Distribution. 4.3.2 Fast Determination of
Molar Mass. 4.3.3 Characterization of Complex Polymers. 4.3.3.1 Branched
Polymers. 4.3.3.2 Copolymers and Polymer Blends. 4.3.4 Conformation Plots.
4.3.5 Mark-Houwink Plots. 4.4 Keynotes. 4.5 References. 5 Asymmetric Flow
Field Flow Fractionation. 5.1 Introduction. 5.2 Theory and Basic
Principles. 5.2.1 Separation Mechanisms. 5.2.2 Resolution and Band
Broadening. 5.3 Instrumentation. 5.4 Measurements and Data Processing.
5.4.1 Influence of Separation Conditions. 5.4.1.1 Isocratic and Gradient
Experiments. 5.4.1.2 Overloading. 5.4.2 Practical Measurements. 5.5 A4F
Applications. 5.6 Keynotes. 5.7 References. 6 Characterization of Branched
Polymers. 6.1 Introduction. 6.2 Detection and Characterization of
Branching. 6.2.1 SEC Elution Behavior of Branched Polymers. 6.2.2
Distribution of Branching. 6.2.3 Average Branching Ratios. 6.2.4 Other
Methods for the Identification and Characterization of Branching. 6.3
Examples of Characterization of Branching. 6.4 Keynotes. 6.5 References.
Symbols. Abbreviations. Index.