Ion Channels: Channel Production and Optical Methods (eBook, ePUB)

• Format: ePub

Produktbeschreibung

Ion Channels Part B, Volume 652 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of topics, including NMDAR, Pannexin, and CALHM, Making NaV1.4 and NaV1.7, TRPVs, Purification native nAChRs, GABAR Radu Aricescu, TRPV5/2, NaV1.5, KATP, TRPA1, TREK-1, SARS-CoV-2 3a ion channel, Ion channel conformational dynamics by encoded unnatural amino acid, Fluorescence lifetime measurement of absolute membrane potential, Fluorescent Toxins as Activity Sensors, FRET Analyses of Ion Channel Protein-Protein Interactions, Control of Ion Channel Gating with Photo-Switchable Tweezers, and Counting Subunits in Kv Channel Complexes.

• Provides the authority and expertise of leading contributors from an international board of authors
• Presents the latest release in the Methods in Enzymology series

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Produktdetails

• Verlag: Elsevier Science & Techn.
• Seitenzahl: 420
• Erscheinungstermin: 5. Juni 2021
• Englisch
• ISBN-13: 9780323853774
• Artikelnr.: 62105357

Autorenporträt

Daniel L. Minor, Professor, Cardiovascular Research Institute, University of California San Francisco, San Francisco, CA, USAHenry M. Colecraft, John C. Dalton Professor of Physiology and Cellular Biophysics & Cellular Biophysics, Columbia University College of Physicians & Surgeons, New York, NY, USA

Inhaltsangabe

Preface



Daniel L. Minor Jr. and Henry M. Colecraft



Methods to produce and purify ion channels



1. Effective production of oligomeric membrane proteins by EarlyBac



Hiro Furukawa



2. Preparation of the Transient Receptor Potential Vanilloid 2 (TRPV2) channel for structural studies



Lejla Zubcevic



3. Production and Purification of TRPV2 and TRPV5 for Cryo-Electron Microscopy Studies



Vera Moiseekova-Bell



4. Sample Preparation of the Human TRPA1 Ion Channel for Cryo-EM Studies



Seok-Yong Lee



5. Expression and Purification of the Cardiac Sodium Channel NaV1.5 for Cryo-EM Structure Determination



William A. Catterall



6. Structural determination of human Nav1.4 and Nav1.7 using single particle cryo-electron microscopy



Nieng Yan



7. Production and Purification of ATP-Sensitive Potassium Channel Particles for Cryo-Electron Microscopy



Show-Ling Shyng



8. Production of K2P2.1 (TREK-1) for structural studies



Daniel L. Minor Jr.



9. Purification of a native nicotinic receptor



Ryan Hibbs



10. SARS-CoV-2 3a expression, purification, and reconstitution into lipid nanodiscs



Stephen Brohawn

Optical methods for probing and controlling channel function



11. Studying Ion Channel Conformation Dynamics by Encoding Coumarin as Unnatural Amino Acid



Sebastian E. Brauchi



12. Fluorescence lifetime measurement of absolute membrane potential



Evan Miller



13. Fluorescent Toxins as Ion Channel Activity Sensors



Jon Sack



14. Probing Ion Channel Macromolecular Interactions using Fluorescence Resonance Energy Transfer



Manu Ben-Johny



15. Manipulation of Ion Channel Gating with Photoswitchable Tweezers



Thomas Grutter



16. Counting Subunits in Kv Channel Complexes



Rikard Blunck