Intelligent Surfaces in Biotechnology (eBook, PDF)

Scientific and Engineering Concepts, Enabling Technologies, and Translation to Bio-Oriented Applications
Redaktion: Grandin, H. Michelle; Textor, Marcus

• Format: PDF

Produktbeschreibung

A comprehensive overview of smart and responsive surfaces in biotechnology and their applications A wave of recent advances in cell biology, biophysics, chemistry, and materials science has enabled the development of a new generation of smart biomaterials. Intelligent Surfaces in Biotechnology: Scientific and Engineering Concepts, Enabling Technologies, and Translation to Bio-Oriented Applications provides readers with a comprehensive overview of surface modifications and their applications, including coverage of the physico-chemical properties, characterization methods, smart coating technologies, and demonstration of performance in vitro and in vivo. The first part of the book covers applications in the fields of biosensing and biodiagnostics, while the second part focuses more on coatings for medical devices, drug delivery, and tailored cell-surface interactions. The book explores intelligent surface applications such as tissue engineering, drug targeting and delivery, wound healing and anti-infection strategies, biosensors, nanopatterning, and bioinspired design of novel responsive materials and multifunctional surfaces. Designed to aid scientists and engineers in understanding the rapidly developing field of biofunctional surfaces, Intelligent Surfaces in Biotechnology is an edited volume with each chapter written by a respected expert and featuring examples taken from the most state-of-the-art developments in the discipline. Cover Image: Design concept for a diagnostic microfluidic system based on responsive polymer- and antibody-conjugated nanobeads (see Chapter 2 of this book, Figure 2.5; reproduced by permission from the Royal Society of Chemistry).

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Produktdetails

• Verlag: John Wiley & Sons
• Seitenzahl: 400
• Erscheinungstermin: 31. Januar 2012
• Englisch
• ISBN-13: 9781118181218
• Artikelnr.: 37360837

Autorenporträt

H. Michelle Grandin currently works as a scientific writer and consultant in the field of tissue regeneration and implant surfaces for dental applications. Previously, she worked as a senior research scientist in the biointerface group of Marcus Textor at ETH Zurich. There, she developed a waveguide fluorescence microscope for the study of bio-interactions in the near-field as well as leading a group in the development of 3D single cellular microwell arrays for application in stem cell and cancer research. Marcus Textor is a Professor Emeritus at ETH Zurich, Department of Materials. He was head of a research group dedicated to surfaces and interfaces in bio-related fields of material science. His research interests cover both fundamental aspects of the behavior of materials in contact with biological milieus and the design and development of surfaces that elicit biospecific responses.

Inhaltsangabe

Foreword xv Preface xix Contributors xxiii 1. Stimulus-Responsive Polymers
as Intelligent Coatings for Biosensors: Architectures, Response Mechanisms,
and Applications 1 Vinalia Tjong, Jianming Zhang, Ashutosh Chilkoti, and
Stefan Zauscher 1.1 Introduction 1 1.2 SRP Architectures for Biosensor
Applications 2 1.2.1 Cross-Linked Polymer Networks (Hydrogels) 2 1.2.2
End-Grafted Polymer Chains (Polymer Brushes) 5 1.2.3 Self-Assembled
Polyelectrolyte (PEL) Multilayers (LBL Thin Films) 5 1.2.4 Molecularly
Imprinted Polymers 6 1.2.5 Hybrid Coatings 6 1.3 Mechanisms of Response 6
1.3.1 Sensing Selectivity 6 1.3.2 Conformational Reorganization of SRP
Coatings 7 1.4 Sensing and Transduction Mechanisms 9 1.4.1 Optical
Transduction 9 1.4.2 Electrochemical Transduction 14 1.4.3 Mechanical
Transduction 17 1.5 Limitations and Challenges 19 1.5.1 LOD and Sensitivity
19 1.5.2 Selectivity 20 1.5.3 Working Range 20 1.5.4 Response Time 20 1.5.5
Reliability and Long-Term Stability 21 1.6 Conclusion and Outlook 22
Acknowledgements 22 References 22 2. Smart Surfaces for Point-of-Care
Diagnostics 31 Michael A. Nash, Allison L. Golden, John M. Hoffman, James
J. Lai, and Patrick S. Stayton 2.1 Introduction 31 2.1.1 POC Testing
Challenges 32 2.2 Standard Methods for Biomarker Purification, Enrichment,
and Detection 33 2.3 Smart Reagents for Biomarker Purification and
Processing 34 2.3.1 IgG Antibody-pNIPAAm Conjugates 38 2.3.2 Single-Chain
Antibody-pNIPAAm Conjugates 39 2.3.3 Nucleotide-pNIPAAm Conjugates 40 2.3.4
Magnetic Nanoparticle (mNP)-pNIPAAm Conjugates 40 2.3.5 Gold Nanoparticle
(AuNP)-pNIPAAm Conjugates 42 2.4 Sample-Processing Modules for Smart
Conjugate Bioassays 44 2.4.1 Grafting of pNIPAAm from Microchannel Surfaces
45 2.4.2 Grafting of pNIPAAm from Porous Membranes 48 2.4.3 Magnetic
Processing Modules 51 2.5 Devices for Use in Smart Conjugate Bioassays 54
2.5.1 Lateral-Flow Immunochromatography Devices 55 2.5.2 Wicking Membrane
Flow-Through Devices 56 2.5.3 Polylaminate Microfl uidic Devices 57 2.5.4
Multilayer PDMS Smart Microfl udic Devices 58 2.6 Conclusions 60 References
61 3. Design of Intelligent Surface Modifications and Optimal Liquid
Handling for Nanoscale Bioanalytical Sensors 71 Laurent Feuz, Fredrik Höök,
and Erik Reimhult 3.1 Introduction 71 3.2 Orthogonal Small (Nano)-Scale
Surface Modification Using Molecular Self-Assembly 75 3.2.1 Surface Anchor:
How to Define and Retain a Molecular Pattern 77 3.2.2 Spacer: How to
Suppress Binding 83 3.2.3 Recognizing and Capturing Analytes on an
Intelligent Nanostructure 86 3.3 Alternative Surface Patterning Strategies
89 3.3.1 Lithographic Patterning of Physisorbed Macromolecules 89 3.3.2
Nanoscale Molecular Surface Modification through Printing 90 3.3.3
Nanoscale Molecular Surface Modification through Direct Writing 91 3.3.4
Multivalency and the Intelligent Fluid Biointerface 92 3.3.5 Summary
Functionalization of Nanoscale Biosensors 95 3.4 The Challenge of Analyte
Transport 95 3.4.1 Convective versus Diffusive Flux ( jC vs. jD) 98 3.4.2
Reactive versus Diffusive Flux ( jR vs. jD) 106 3.4.3 Design and Operation
Criteria for Efficient Mass Transport 108 3.5 Concluding Remarks 112
References 113 4. Intelligent Surfaces for Field-Effect Transistor-Based
Nanobiosensing 123 Akira Matsumoto, Yuji Miyahara, and Kazunori Kataoka 4.1
Introduction 123 4.2 FET-Based Biosensors 124 4.2.1
Metal-Insulator-Semiconductor (MIS) Capacitors 124 4.2.2 Principles of
bio-FETs 125 4.2.3 Ion-Sensitive Field-Effect Transistors (ISFETs) and
Their Direct Coupling with Various Biorecognition Elements as a
Conventional Approach to bio-FETs 126 4.3 Intelligent Surfaces for Signal
Transduction and Amplification of bio-FETs 128 4.3.1 CNT-Mediated Signal
Transduction 128 4.3.2 SAM-Assisted Detection 129 4.3.3 Stimuli-Responsive
Polymer Gel-Based Interfaces for "Debye Length-Free" Detection 130 4.4 New
Targets of bio-FETs 132 4.4.1 Carbohydrate Chain Sialic Acid (SA) Detection
Using PBA SAM-Modifi ed FETs 132 4.4.2 Scent Detection Using "Beetle/Chip"
FETs 134 4.4.3 Aptamer-Modifi ed Biorecognition Surfaces for a Universal
Platform of bio-FETs 134 4.5 Future Perspective 135 References 136 5.
Supported Lipid Bilayers: Intelligent Surfaces for Ion Channel Recordings
141 Andreas Janshoff and Claudia Steinem 5.1 Introduction 141 5.2 Supported
Lipid Bilayers 142 5.2.1 SSMs on Flat Interfaces 142 5.2.2 SSMs on
Porous/Aperture Containing Surfaces 146 5.2.3 Patterning of SSMs 148 5.3
Characteristics of SSMs 151 5.3.1 Thermomechanical Properties of SSMs 151
5.3.2 Mechanical Stability 154 5.4 Ion Channels in SSMs 157 5.4.1 Carriers
158 5.4.2 Channel-Forming Peptides 158 5.4.3 Channel-Forming Proteins 162
5.5 Future Perspective: Ion Channels in Micropatterned Membranes 163
References 172 6. Antimicrobial and Anti-Inflammatory Intelligent Surfaces
183 Hans J. Griesser, Heike Hall, Toby A. Jenkins, Stefani S. Griesser, and
Krasimir Vasilev 6.1 Introduction 183 6.2 Antibacterial Strategies 184
6.2.1 The Infection Problem 184 6.2.2 Approaches to Antibacterial Device
Surfaces 186 6.2.3 Release of Antimicrobial Compounds from Polymers and
Polymeric Coatings 190 6.2.4 Silver-Releasing Coatings 191 6.2.5 Nonfouling
Coatings 196 6.2.6 Surface-Grafted Antibacterial Molecules 196 6.3
Bioactive Antibacterial Surfaces 198 6.3.1 Established, Commercially
Available Antibiotics 198 6.3.2 Experimental Antibiotics 201 6.4
Stimulus-Responsive Antibacterial Coatings for Wound Dressings 204 6.5
Anti-Infl ammatory Surfaces 208 6.5.1 The Infl ammatory Response 208 6.5.2
Contact Activation of the Complement System 209 6.5.3 Foreign Body Reaction
211 6.5.4 Anti-infl ammatory Medication 212 6.5.5 Local Prevention of the
Infl ammatory Reaction on Medical Device/Implant Surfaces 215 6.6
Conclusions and Outlook 224 References 226 7. Intelligent Polymer Thin
Films and Coatings for Drug Delivery 243 Alexander N. Zelikin and Brigitte
Städler 7.1 Introduction 243 7.2 Surface-Mediated Drug Delivery 246 7.2.1
Controlled Cell Adhesion and Proliferation 247 7.2.2 Small Cargo 254 7.2.3
Delivery and Presentation of Protein and Peptide Cargo 257 7.2.4 Delivery
of Gene Cargo 261 7.3 Drug Delivery Vehicles with Functional Polymer
Coatings 268 7.3.1 Core-Shell Particles 268 7.3.2 Polymer Capsules 271 7.4
Concluding Remarks 280 References 280 8. Micro- and Nanopatterning of
Active Biomolecules and Cells 291 Daniel Aydin, Vera C.
Hirschfeld-Warneken, Ilia Louban, and Joachim P. Spatz 8.1 Introduction 291
8.2 Chemical Approaches for Protein Immobilization 291 8.3 Biomolecule
Patterning by "Top-Down" Techniques 294 8.3.1 Microcontact Printing (1/4CP)
294 8.3.2 Nanoimprint Lithography (NIL) 294 8.3.3 Electron Beam Lithography
(EBL) 295 8.3.4 Dip-Pen Nanolithography (DPN) 295 8.4 Biomolecule
Nanoarrays by Block Copolymer Nanolithography 296 8.4.1 Block Copolymer
Nanolithography 297 8.4.2 Biofunctionalization of Nanostructures 299 8.4.3
Hierarchically Nanostructured Biomolecule Arrays 300 8.4.4 Fabrication of
Nanoscale Distance Gradients 302 8.4.5 Soft Polymeric Biomolecule Arrays
303 8.5 Application of Nanostructured Surfaces to Study Cell Adhesion 305
8.5.1 Mimicking the Extracellular Environment 305 8.5.2 Nanoscale Control
of Cellular Adhesion 305 8.5.3 Micro-Nanopatterns to Uncouple Local from
Global Density 307 8.5.4 Nanoscale Gradients to Induce Cell Polarization
and Directed Migration 309 8.5.5 Substrate Elasticity Determines Cell Fate
311 8.6 Conclusion 313 References 313 9. Responsive Polymer Coatings for
Smart Applications in Chromatography, Drug Delivery Systems, and Cell Sheet
Engineering 321 Rogério P. Pirraco, Masayuki Yamato, Yoshikatsu Akiyama,
Kenichi Nagase, Masamichi Nakayama, Alexandra P. Marques, Rui L. Reis, and
Teruo Okano 9.1 Introduction 321 9.2 Temperature-Responsive Chromatography
322 9.2.1 Hydrophobic Chromatography 322 9.2.2 Ion-Exchange Chromatography
324 9.2.3 Affinity Chromatography 327 9.3 Temperature-Responsive Polymer
Micelles 328 9.3.1 Temperature-Responsive Corona 329 9.3.2
Temperature-Responsive Core 332 9.4 Temperature-Responsive Culture Surfaces
333 9.4.1 Temperature-Responsive Culture Dishes 333 9.4.2
Temperature-Responsive Surfaces on Porous Substrates 336 9.4.3
Functionalization of Temperature-Responsive Surfaces 336 9.4.4
Temperature-Responsive Surface Patterning 338 9.5 Cell Sheet Engineering
339 9.5.1 Characterization of Harvested Cell Sheets 339 9.5.2 Applications
in Regenerative Medicine 340 9.5.3 Thick Tissue Reconstruction 343 9.6
Conclusions 345 References 346 Index 355