Bernard A. Olsen, Brian W. Pack

Hydrophilic Interaction Chromatography (eBook, PDF)

A Guide for Practitioners

• Format: PDF

Produktbeschreibung

Discover how to use HILIC to analyze and better understand polar compounds An increasingly popular analytical method, hydrophilic interaction chromatography (HILIC) has the ability to retain and separate polar compounds that are often difficult to analyze by reversed-phase high-performance liquid chromatography (HPLC) or other analytical methods. Offering a comprehensive review, this book enables readers to develop a fundamental understanding of how HILIC works and then apply that knowledge to develop and implement a variety of practical applications. Hydrophilic Interaction Chromatography begins with discussions of HILIC retention mechanisms, stationary phases, and general method development. This sets the foundation for the book's extensive coverage of applications. The authors address unique separation challenges for bioanalytical, environmental, pharmaceutical, and biochemical applications. Moreover, there is a thorough discussion of HILIC in two-dimensional chromatography. With contributions from leading analytical scientists who have extensive experience in HILIC as well as HPLC, Hydrophilic Interaction Chromatography serves as a practical guide for researchers, featuring: * Detailed examples of HILIC methods and development approaches * Thorough explanations of retention mechanisms and the impact of stationary phase and mobile phase properties on separations * Step-by-step guidance for developing efficient, sensitive, and robust HILIC methods * References to the primary literature at the end of each chapter Hydrophilic Interaction Chromatography is written for scientists who use or develop analytical methods for the separation of polar compounds. In particular, these researchers will discover how HILIC can be used to analyze and better understand the composition of pharmaceutical, bioanalytical, biochemical, chemical, food, and environmental samples.

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Produktdetails

• Verlag: John Wiley & Sons
• Seitenzahl: 336
• Erscheinungstermin: 14. Januar 2013
• Englisch
• ISBN-13: 9781118495216
• Artikelnr.: 37350803

Autorenporträt

BERNARD A. OLSEN, PhD, has close to three decades of experience at Eli Lilly and Company in chemistry, manufacturing, and control of drug substances and drug products. He contributed to the development and support of more than twenty-five commercialized drugs as well as numerous developmental drugs. A Fellow of the American Association of Pharmaceutical Scientists, Dr. Olsen currently provides consulting and training services to the pharmaceutical industry and serves as Chair of the USP Expert Committee on Monograph Development: Small Molecules 3. BRIAN W. PACK, PhD, is a Research Advisor in Analytical Sciences R&D at Eli Lilly and Company, where he has contributed to the development of many solid oral and parenteral dosage forms. He is recognized for his contributions to HPLC method development, raw material control strategies, genotoxic impurities, cleaning validation, and colorimetry. Dr. Pack has published on the topics of HILIC applications, Raman spectroscopy, mass spectrometry, dissolution, and cleaning verification.

Inhaltsangabe

PREFACE xiii CONTRIBUTORS xv CHAPTER 1 SEPARATION MECHANISMS IN HYDROPHILIC
INTERACTION CHROMATOGRAPHY 1 David V. McCalley 1.1 Introduction 1 1.2
Historical Background: Recognition of the Contribution of Partition, Ion
Exchange, and RP Interactions to the Retention Process 3 1.3 Recent Studies
on the Contributory Mechanisms to HILIC Retention 7 1.4 Conclusions 38
References 38 CHAPTER 2 STATIONARY PHASES FOR HILIC 43 Mohammed E.A.
Ibrahim and Charles A. Lucy 2.1 Introduction 43 2.2 HILIC Stationary Phases
44 2.3 Commercial HILIC Phases 61 2.4 Conclusions 77 Acknowledgments 77
References 77 CHAPTER 3 HILIC METHOD DEVELOPMENT 87 Yong Guo and Xiande
Wang 3.1 Introduction 87 3.2 General Method Development Considerations 88
3.3 HILIC Method Development 93 3.4 Detection for HILIC Methods 104 3.5
Conclusions 107 References 108 CHAPTER 4 PHARMACEUTICAL APPLICATIONS OF
HYDROPHILIC INTERACTION CHROMATOGRAPHY 111 Bernard A. Olsen, Donald S.
Risley, V. Scott Sharp, Brian W. Pack, and Michelle L. Lytle 4.1
Introduction 112 4.2 Determination of Counterions 117 4.3 Main Component
Methods 129 4.4 Determination of Impurities 135 4.5 Excipients 146 4.6
Chiral Applications 152 4.7 Conclusions 161 References 161 CHAPTER 5
HYDROPHILIC INTERACTION CHROMATOGRAPHY (HILIC) FOR DRUG DISCOVERY 169
Alfonso Espada and Mark Strege 5.1 Drug Discovery Model 169 5.2 HILIC
Applications for In Vitro Biology 170 5.4 Practical Considerations 186 5.5
Conclusions 187 References 188 CHAPTER 6 ADVANCES IN HYDROPHILIC
INTERACTION CHROMATOGRAPHY (HILIC) FOR BIOCHEMICAL APPLICATIONS 195 Fred
Rabel and Bernard A. Olsen 6.1 Introduction 195 6.2 Carbohydrates 196 6.2.1
Mono- and Disaccharides 196 6.3 Nucleobases and Nucleosides 203 6.4
Oligonucleotides 205 6.5 Amino Acids and Peptides 206 6.6 Proteins 209 6.7
Phospholipids 209 6.8 Conclusions 211 References 212 CHAPTER 7 HILIC-MS FOR
TARGETED METABOLOMICS AND SMALL MOLECULE BIOANALYSIS 219 Hien P. Nguyen,
Heather D. Tippens, and Kevin A. Schug 7.1 Introduction 219 7.2 The Role of
HILIC-MS in Targeted Metabolomics versus Other LC Modes 221 7.3 Strategies
for Method Development Based on Retention Behavior of Targeted Metabolites
on HILIC Stationary Phases 223 7.4 Summary 231 Acknowledgments 232
References 232 CHAPTER 8 HILIC FOR FOOD, ENVIRONMENTAL, AND OTHER
APPLICATIONS 239 Michael A. Koupparis, Nikolaos C. Megoulas, and Aikaterini
M. Gremilogianni 8.1 Introduction 239 8.2 Food Applications for HILIC 240
8.3 Environmental and Other Applications of HILIC 254 8.4 Conclusions 257
References 259 CHAPTER 9 THEORY AND PRACTICE OF TWO-DIMENSIONAL LIQUID
CHROMATOGRAPHY SEPARATIONS INVOLVING THE HILIC MODE OF SEPARATION 265
Stephen R. Groskreutz and Dwight R. Stoll 9.1 Fundamentals of
Multidimensional Liquid Chromatography 265 9.2 Complementarity of HILIC
Selectivity to Other Separation Modes 278 9.3 Instrumentation and
Experimental Considerations 278 9.4 Applications 291 9.5 The Future of
HILIC Separations in 2DLC 298 References 298 INDEX 307

Rezensionen

"The well-balanced mixture with updated HILIC theory and detailed practical descriptions makes it a useful resource for scientists working in analytical chemistry, biochemistry, biotechnology, and bioinformatics, and a valuable resource for people in the analytical column industry." (Analytical and Bioanalytical Chemistry, 22 September 2013)