Gene Delivery to Mammalian Cells (eBook, PDF)

Volume 2: Viral Gene Transfer Techniques
Redaktion: Heiser, William C.

• Format: PDF

Produktbeschreibung

Whether to assess the function of new genes identified from the Human Genome Project or to apply gene therapy successfully, it is often necessary to deliver genes to specific cells. In Gene Delivery to Mammalian Cells, highly experienced researchers describe in great detail methods that have proven most useful in delivering genes to mammalian cells. Volume 2: Viral Gene Transfer Techniques details procedures for delivering genes to cells in vitro and in vivo, including the use of lentiviral vectors, adenovirus, adeno-associated viruses, alphavirus, herpes simplex virus, baculovirus, and retrovirus. Many of these techniques have only been in practice for a few years and are still being refined and updated. Some are being used not only in basic science, but also in gene therapy applications. Each protocol contains step-by-step instructions, along with background notes, equipment and reagent lists, and tips on troubleshooting and avoiding known pitfalls. Introductory chapters review the delivery methods presented, discussing their advantages and disadvantages, how they have been used successfully for gene delivery, and the future of their technology. An accompanying volume, Volume 1: Nonviral Gene Transfer Techniques, focuses on gene delivery by a variety of chemical and physical methods. Comprehensive and cutting-edge, Gene Delivery to Mammalian Cells, volumes 1 and 2, offers biomedical specialists in industry and academia an unrivaled collection of reliable methods for the chemical, physical, and viral delivery of genes to mammalian cells, all prepared to save laboratory time and ensure experimental success.

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Produktdetails

• Verlag: Humana Press
• Seitenzahl: 566
• Erscheinungstermin: 2. Februar 2008
• Englisch
• ISBN-13: 9781592596508
• Artikelnr.: 64693768

Inhaltsangabe

Delivery Using Adenoviruses.- Adenovirus-Mediated Gene Delivery.- DNA Delivery to Cells in Culture.- Adenovirus-Mediated Gene Delivery to Skeletal Muscle.- Delivery of Adenoviral DNA to Mouse Liver.- Delivery of DNA to Lung Airway Epithelium.- Delivery of DNA to Pulmonary Endothelium Using Adenoviral Vectors.- Gene Transfer to Brain and Spinal Cord Using Recombinant Adenoviral Vectors.- Adenovirus-Mediated Gene Transfer to Tumor Cells.- Adenovirus-Mediated Gene Delivery to Dendritic Cells.- Delivery Using Adeno-Associated Viruses.- Overview of Adeno-Associated Viral Vectors.- AAV Vector Delivery to Cells in Culture.- AAV-Mediated Gene Transfer to Skeletal Muscle.- AAV-Mediated Gene Transfer to the Liver.- AAV-Mediated Gene Transfer to Mouse Lungs.- Gene Delivery to the Mammalian Heart Using AAV Vectors.- Gene Delivery to the Mouse Brain with Adeno-Associated Virus.- Delivery of DNA to Tumor Cells In Vivo Using Adeno-Associated Virus.- Gene Delivery to Human and Murine Primitive Hematopoietic Stem and Progenitor Cells by AAV2 Vectors.- Delivery Using Herpes Simplex Viruses.- Delivery Using Herpes Simplex Virus.- Gene Transfer to Skeletal Muscle Using Herpes Simplex Virus-Based Vectors.- Delivery of Herpes Simplex Virus-Based Vectors to the Nervous System.- Gene Transfer to Glial Tumors Using Herpes Simplex Virus.- Delivery of Herpes Simplex Virus-Based Vectors to Stem Cells.- Delivery Using Baculoviruses.- Baculovirus-Mediated Gene Delivery into Mammalian Cells.- Delivery Using Lentiviruses.- Gene Delivery by Lentivirus Vectors.- Lentiviral Vectors for the Delivery of DNA into Mammalian Cells.- Stable Gene Delivery to CNS Cells Using Lentiviral Vectors.- Gene Delivery to Hematopoietic Stem Cells Using Lentiviral Vectors.- Delivery of Genes to the Eye Using Lentiviral Vectors.- Lentiviral Transduction of Human Dendritic Cells.- Delivery Using Retrovirus.- Gene Transfer by Retroviral Vectors.- Gene Delivery to Cells in Culture Using Retroviruses.- Retrovirus-Mediated Gene Transfer to Tumors.- Dilivery of Genes to Hematopoietic Stem Cells.- Delivery Using Alphaviruses.- Delivery and Expression of Heterologous Genes in Mammalian Cells Using Self-Replicating Alphavirus Vectors.

Rezensionen

For Volumes 1 & 2

"This unrivaled collection of proven, reliable methods for the chemical, physical, and viral delivery of genes into mammalian cells, presented in great detail by experienced researchers, should be a valuable reference work for novices in this filed and experienced researchers alike." - Medical Oncology

"...clearly and succinctly presented...a must for any biology or medical library." - ASM News