Eukaryotic Cell Cultures (eBook, PDF)

Basics and Applications
Redaktion: Acton, Ronald T.

• Format: PDF

Produktbeschreibung

The Second International Cell Culture Congress was structured as was the First Congress to bring together scientists from academia and industry to discuss the use of cell culture in support of bioscience. It was felt that a forum whereby state-of the-art presentations were followed by informal workshops would provide opportunity for the greatest exchange of information. Within the atmosphere of the workshop, problems common to basic as well as applied research were discussed and directions for the future were brought to light. These proceedings reflect and epitomize those discussions. Although it is difficult to cover all scientific disciplines utilizing cells in culture, we feel key areas were addressed at the Congress and are herein presented. Considerable emphasis has been given to the methods for establishing cells in culture and characterizing the cells once established as well as the improved technology for growing established cell lines. Examples of how recombinant DNA technology is being used to manipulate genes within mammalian cells, to clone mammalian genes and to insert them in prokaryotes has been included. Major emphasis has been given to the use of lymphocytes in culture for understanding immune responsiveness and the culturing of a variety of cell types as a means to understand disease states.

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Produktdetails

• Verlag: Springer New York
• Seitenzahl: 556
• Erscheinungstermin: 9. März 2013
• Englisch
• ISBN-13: 9781461593768
• Artikelnr.: 44059581

Inhaltsangabe

Establishing, Characterizing and Growing Cells in Culture.- Establishing and Characterizing Cells in Culture.- Cell Characterization by use of Multiple Genetic Markers.- Characteristics of Lympho-Myeolopietic Stem Cells Isolated from Canine Peripheral Blood.- Growth Characteristics of A431 Human Epidermoid Carcinoma Cells in Serum-Free Medium: Inhibition by Epidermal Growth Factor.- In Vitro Senescence, Differentiated Function, and Transformation in Cultured Vascular Endothelial Cells.- Cytogenetics Effects of Cyclamates.- Mammalian Cell Culture: Technology and Physiology.- A High Efficiency Stirrer for Suspension Cell Culture with or without Microcarriers.- Alternative Surfaces for Microcarrier Culture of Animal Cells.- Products of Cells In Culture.- Interferon Production in Micrcarrier Culture of Human Fibroblast Cells.- In Vitro and In Vivo Effects of Endothelial Cell-Derived Growth Factor.- Lymphotoxins - A Multicomponent System of Growth Inhibitory and Cell-Lytic Glycoproteins.- Production of Gamma (Immune) Interferon by a Permanent Human T-Lymphocyte Cell Line.- Thymic Inhibition of Myelopoietic Proliferation.- Problems in the Bioassay of Products from Cultures HEK Cells: Plasminogen Activator.- A Simple Methodology for the Routine Production and Partial Purification of Human Lymphoblastoid Interferon.- Use of Recombinant DNA Technology.- The Cloning Isolation and Characterization of a Biologically Active Human Enzyme, Urokinase, in E. Coli.- Cloning of the Rat Endogenous Helper Leukemia Virus DNA Sequence and Expression of the Helper Activity Encoded by the Cloned DNA Sequence in Normal Rat Kidney Cells by Microinjection.- Analysis of CAD Gene Amplification using a Combined approach of Molecular Genetics and Cytogenetics.- Lymphocytes in Culture: A System forUnderstanding Immune Responsiveness.- Alloreactive T Cell Clones.- Productive Murine Leukemia Virus (MuLV) Infection of EL4 T-Lymphoblastoid Cells: Selective Elevation of H-2 Surface Expression and Possible Association of Thy-1 Antigen with Viruses.- Construction of Human T-Cell Hybrids with Helper Function.- Cultured Human T Lymphocyte Lines and Clones as Immunogenetic Tools.- Use of Cells In Culture to Study Disease.- Long-term Persistance in Experimental Animals of Components of Skin-Equivalent Grafts Fabricated in the Laboratory.- Angiotensin Receptors and the Control of Na + and K + Transport in Cultured Aortic Smooth Muscle and Brain Microvessel Cells.- Antigenic Expression of Human Melanoma Cells in Serum-Free Medium.- Use of Cell Culture to Identify Human Precancer.- Uses of Tissue Culture and Cryopreservation in Pancreatic Islet Transplantation.- Mechanisms of Mediator Release from Neutrophils.