Serban Moldoveanu, Victor David

Essentials in Modern HPLC Separations (eBook, ePUB)

• Format: ePub

Produktbeschreibung

Essentials in Modern HPLC Separations discusses the role of separation in high performance liquid chromatography (HPLC). This up-to-date reference systematically covers new developments in types and characteristics of stationary phases, mobile phases, and other factors of this technique that influence separation of compounds being analyzed. The volume also considers the selection process for stationary and mobile phases in relation to the molecules being separated and examined, as well as their matrices.

The book includes a section on the contemporary applications of HPLC, particularly the analysis of pharmaceutical and biological samples, food and beverages, environmental samples, and more.

• Discusses key parameters in HPLC separation
• Describes interrelation between various HPLC features (solvent pressure, separation, detection)
• Includes a large number of references

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Produktdetails

• Verlag: Elsevier Science & Techn.
• Seitenzahl: 550
• Erscheinungstermin: 8. November 2012
• Englisch
• ISBN-13: 9780123850140
• Artikelnr.: 47274583

Autorenporträt

Dr. Serban C. Moldoveanu is Senior Principal Scientist at R. J. Reynolds Tobacco Company. His research activity is focused on various aspects of chromatography including method development for the analysis by GC/MS, HPLC, and LC/MS/MS of natural products and cigarette smoke. He has also performed research on pyrolysis of a variety of polymers and small molecules. He has over 100 publications in peer reviewed journals, eleven books, and several chapter contributions. He is a member of the editorial board of the Journal of Analytical Methods in Chemistry.

Inhaltsangabe

Part 1. Basic information about HPLC1. Introductory information regarding HPLC 2. Overview of HPLC instrumentation and its use3. Parameters for the characterization of HPLC separation4. Equilibrium types in HPLC5. Intermolecular forces6. Characterization of analytes and matrices 7. Mobile phases and their properties8. Analytical HPLC columns and their characteristics

Part 2. Main types of HPLC separations 9. Reversed-phase HPLC10. Other HPLC separations performed on hydrophobic stationary phases11. Hydrophilic interaction chromatography (HILIC)12. Other HPLC separations performed on polar stationary phases13. Ion-exchange, ion-moderated, and ligand-exchange liquid chromatography14. Chiral HPLC Separations15. Size exclusion HPLC16. Affinity, immunoaffinity, and aptamer type HPLC17. Mixed mode HPLC

Part 3. Practice of HPLC analysis18. Utilization of HPLC in chemical analysis

Part 4. Appendices