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Edited by one of the leading experts in the field, this book fills the need for a book presenting the most important methods for high-throughput screenings and functional characterization of enzymes. It adopts an interdisciplinary approach, making it indispensable for all those involved in this expanding field, and reflects the major advances made over the past few years. For biochemists, analytical, organic and catalytic chemists, and biotechnologists.
Edited by one of the leading experts in the field, this book fills the need for a book presenting the most important methods for high-throughput screenings and functional characterization of enzymes. It adopts an interdisciplinary approach, making it indispensable for all those involved in this expanding field, and reflects the major advances made over the past few years. For biochemists, analytical, organic and catalytic chemists, and biotechnologists.
1985 Jean-Louis Reymond obtained his diploma working with Professor Seebach at the ETH Zürich. He finished his thesis in the field of total synthesis in 1989 in the group of Professor Vogel. After being in the United States for two years at the Scripps Research Institute he became an assistant professor there. 1997 he returned to the University of Bern, Switzerland. His research interest is biocatalysis and in particular enzyme design, assays, profiling, fingerprints and inhibitors.
Inhaltsangabe
PART I. THE CHEMISTRY OF ENZYME ASSAYS 1. Chromogenic Assay of Hydrolases for Activity and Enantioselectivity 2. High-throughput screening systems for assaying the enantioselectivity of enzymes 3. High throughput screening methods developed for oxidoreductases 4. Industrial perspective on assays PART II. ENZYME ASSAYS AND GENETIC SELECTION 5. Agar-plate based assays 6. Genetic selection for biocatalysts 7. Three-hybrid systems and in vivo selection 8. Nucleic-acid based approaches on the screening of enzymes. PART III. ENZYME PROFILING 9. Fluorescent probes for lipolytic enzymes 10. Enzyme fingerprinting 11. Protease profiling 12. Enzyme assays on chips INDEXPART I. THE CHEMISTRY OF ENZYME ASSAYS 1. Chromogenic Assay of Hydrolases for Activity and Enantioselectivity 2. High-throughput screening systems for assaying the enantioselectivity of enzymes 3. High throughput screening methods developed for oxidoreductases 4. Industrial perspective on assays PART II. ENZYME ASSAYS AND GENETIC SELECTION 5. Agar-plate based assays 6. Genetic selection for biocatalysts 7. Three-hybrid systems and in vivo selection 8. Nucleic-acid based approaches on the screening of enzymes. PART III. ENZYME PROFILING 9. Fluorescent probes for lipolytic enzymes 10. Enzyme fingerprinting 11. Protease profiling 12. Enzyme assays on chips INDEX
PART I. THE CHEMISTRY OF ENZYME ASSAYS 1. Chromogenic Assay of Hydrolases for Activity and Enantioselectivity 2. High-throughput screening systems for assaying the enantioselectivity of enzymes 3. High throughput screening methods developed for oxidoreductases 4. Industrial perspective on assays PART II. ENZYME ASSAYS AND GENETIC SELECTION 5. Agar-plate based assays 6. Genetic selection for biocatalysts 7. Three-hybrid systems and in vivo selection 8. Nucleic-acid based approaches on the screening of enzymes. PART III. ENZYME PROFILING 9. Fluorescent probes for lipolytic enzymes 10. Enzyme fingerprinting 11. Protease profiling 12. Enzyme assays on chips INDEXPART I. THE CHEMISTRY OF ENZYME ASSAYS 1. Chromogenic Assay of Hydrolases for Activity and Enantioselectivity 2. High-throughput screening systems for assaying the enantioselectivity of enzymes 3. High throughput screening methods developed for oxidoreductases 4. Industrial perspective on assays PART II. ENZYME ASSAYS AND GENETIC SELECTION 5. Agar-plate based assays 6. Genetic selection for biocatalysts 7. Three-hybrid systems and in vivo selection 8. Nucleic-acid based approaches on the screening of enzymes. PART III. ENZYME PROFILING 9. Fluorescent probes for lipolytic enzymes 10. Enzyme fingerprinting 11. Protease profiling 12. Enzyme assays on chips INDEX
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