DNA Sequencing Protocols (eBook, PDF)

Redaktion: Graham, Colin A; Hill, Alison J. M.

• Format: PDF

Produktbeschreibung

Since the publication of the first edition of DNA Sequencing Protocols in 1993, major advances in its technology have made PCR-based semiautomated fluorescent sequencing the norm and enabled the complete sequencing of complex genomes. In DNA Sequencing Protocols, 2nd Edn., Colin Graham and a team of leading investigators and expert clinical scientists update the first edition with a collection of powerful, up-to-date PCR-based methods for DNA sequencing, many suitable for human genome sequencing and mutation detection in human disease. Each method provides detailed, step-by-step instructions to ensure successful results, and includes helpful notes identifying and addressing potential problems. This second edition offers new material on automated DNA sequencers, capillary DNA sequencers, heterozygote mutation detection, web-based sequencing databases and genome sequencing sites, and the human genome project. State-of-the-art and highly practical, DNA Sequencing Protocols, 2nd Edn. constitutes an essential laboratory handbook for geneticists and molecular biologists, offering concise, easy-to-follow methods that will impact today's genome sequencing projects, improve the accuracy and quality of DNA sequences obtained by smaller laboratories, and help lay the foundation for molecular diagnostics.

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Produktdetails

• Verlag: Humana Press
• Seitenzahl: 244
• Erscheinungstermin: 5. Februar 2008
• Englisch
• ISBN-13: 9781592591138
• Artikelnr.: 64693335

Inhaltsangabe

to DNA Sequencing.- The Universal Primers and the Shotgun DNA Sequencing Method.- M13 Sequencing.- Primer Design and Primer-Directed Sequencing.- Direct Sequencing of DNA Produced in a Polymerase Chain Reaction.- Solid Phase Fluorescent Sequencing of the CFTR Gene.- Shotgun DNA Sequencing.- Cycle Sequencing.- Cycle Sequencing of Polymerase Chain Reaction-Amplified Genomic DNA with Dye-Labeled Universal Primers.- Automated Fluorescent DNA Sequencing on the ABI PRISM 377.- Automated Fluorescent DNA Sequencing on the ABI PRISM 310 Genetic Analyzer.- Fluorescent Sequencing Protocols for the ALF.- Fluorescent Sequencing for Heterozygote Mutation Detection.- Sequence Databases and the Internet.- DNA Sequencing by Capillary Array Electrophoresis.

Rezensionen

From Reviews of the First Edition

"...continues the standard of excellent laboratory manuals that has been set by earlier volumes...Many of the protocols are significantly easier to follow than comparable protocols in other lab handbooks...provides one of the best collections of manuals for DNA sequencing applications..." -FEBS Letters
"...a valuable addition to the library of any laboratory that is actively engaged in DNA sequencing..." -Quarterly Review of Biology
"...any laboratory undertaking a large DNA sequencing project...would benefit from having this manual..." -Trends in Biotechnology