Cilia: Structure and Motility (eBook, ePUB)

• Format: ePub

Produktbeschreibung

Along with its companion volume on intraflagellar transport, this book provides researchers with a comprehensive and up-to-date source of methods for the analysis cilia and flagella, focusing primarily on approaches that have been devised or significantly extended since the last volume of Methods in Cell Biology on this topic (volume 47, 1995). Edited by Stephen M. King and Gregory J. Pazour, the newest installment of this highly acclaimed serial will serve as an essential addition to the study of cilia and flagella.

* Covers protocols for cilia and flagella across systems and species * Both classic and state-of-the-art methods readily adaptable across model systems, and designed to last the test of time * Relevant to clinicians interested in respiratory disease, male infertility, and other syndromes who need to learn biochemical, molecular, and genetic approaches to studying cilia, flagella, and related structures

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Produktdetails

• Verlag: Elsevier Science & Techn.
• Seitenzahl: 344
• Erscheinungstermin: 27. November 2009
• Englisch
• ISBN-13: 9780080961064
• Artikelnr.: 38014451

Inhaltsangabe

1. RNAi approaches to axonemal motor function in trypanosomes (Hill) 2.
Homologous recombination to knock out dynein genes in Tetrahymena (Gaertig)
3. RNAi in Chlamydomonas (Cerutti) 4. Planarians as a model system for
analysis of ciliary assembly/motility (Rompolas) 5. Role of nucleotides in
dynein function (Shingyoji) 6. Protein modification to probe dynein
interactions + purification and identification of crosslinked dynein
products (Sakato) 7. Analysis of redox-sensitive dynein components
(Wakabayashi) 8. Purification of dyneins from Chlamydomonas and analysis of
dynein-dynein linkers (Kamiya/King) 9. Kinase/phosphatase-mediated control
of dynein function (Sale) 10. Central pair MT complex and associated
kinesins (Mitchell) 11. Calcium regulation of axonemal function In vitro
motility assays (Smith) 12. Identification and analysis of dynein
regulatory complex components (Porter) 13. Isolation and analysis of radial
spoke proteins (Yang) 14. Purification of dyneins from other model
organisms: Ciona, sea urchin, fish (Inaba) 15. Rescue of mutant phenotypes
by protein electroporation (Kamiya Lab) 16. Tubulin/Dynein interactions
(Raff) 17. Measurement of beat frequency and swimming velocity Cell model
reactivation in vitro assays of dynein function (Kamiya) 18. Live imaging
of ependymal cilia (Lechtreck/Witman) 19. Immunogold labeling of axonemal
components in situ + flat embedding (Geimer) 20. CryoEM approaches to
axonemal organization (Nicastro) 21. CryoEM of dynein-microtubule complexes
(Oda/Kikkawa) 22. Bioinformatic approaches to dynein HC classification
(Yagi) 23. Bioinformatics of non-motor dynein components (Asai) 24.
Biophysical approaches to dynein mechanism (Sutoh) 25. Chlamydomonas
flagellar beat analysis (Foster) 26. Biophysical measurements of motor
function (step size/force production) SAXS of axonemes (Toba/Oiwa)
Cryo-Electron Microscope Tomography to Study Axonemal
Organization
Daniela Nicastro
2. Electron Microscopic Imaging and Analysis of Isolated
Dynein Particles
Anthony J. Roberts and Stan A. Burgess
3. Immunogold Labeling of Flagellar Components In Situ
Stefan Geimer
4. Scanning Electron Microscopy to Examine Cells and Organs
Jovenal T. SanAgustin, John A. Follit, Gregory Hendricks, and Gregory J.
Pazour
5. X-ray Fiber Diffraction Studies on Flagellar Axonemes
Kazuhiro Oiwa, Shinji Kamimura, and Hiroyuki Iwamoto
6. Markers for Neuronal Cilia
Jacqueline S. Domire and Kirk Mykytyn
7. Immunofluorescence Staining of Ciliated Respiratory Epithelial Cells
Heymut Omran and Niki T. Loges
8. Immunoprecipitation to Examine Protein Complexes
Gregory J. Pazour
9. Tandem Affinity Purification of Ciliopathy-Associated Protein
Complexes
Karsten Boldt, Jeroen van Reeuwijk, Christian Johannes Gloeckner,
Marius Ueffing, and Ronald Roepman
10. Crosslinking Methods: Purification and Analysis of Crosslinked Dynein
Products
Miho Sakato
11. Analysis of the Ciliary/Flagellar Beating of Chlamydomonas
Kenneth W. Foster
12. Assays of Cell and Axonemal Motility in Chlamydomonas reinhardtii
Ritsu Kamiya
13. High-Speed Digital Imaging of Ependymal Cilia in the Murine Brain
Karl-Ferdinand Lechtreck, Michael J. Sanderson, and George B. Witman
14. Observation of Nodal Cilia Movement and Measurement of Nodal Flow
Yasushi Okada and Nobutaka Hirokawa
15. Modification of Mouse Nodal Flow by Applying Artificial Flow
Shigenori Nonaka
16. Measuring Cilium-Induced Ca2þ Increases in Cultured Renal Epithelia
Helle A. Praetorius