Cell-free Protein Synthesis (eBook, PDF)

Methods and Protocols

• Format: PDF

Produktbeschreibung

With its detailed description of membrane protein expression, high-throughput and genomic-scale expression studies, both on the analytical and the preparative scale, this book covers the latest advances in the field. The step-by-step protocols and practical examples given for each method constitute practical advice for beginners and experts alike.

Produktdetails

• Verlag: Wiley-VCH
• Seitenzahl: 262
• Erscheinungstermin: 8. September 2008
• Englisch
• ISBN-13: 9783527622696
• Artikelnr.: 42016405

Autorenporträt

Alexander Spirin is a Professor at the Moscow State University, and the director of the Protein Research Institute of the Russian Academy of Sciences in Pushchino, Russia. He is one of the pioneers in the biochemical analysis of protein synthesis and has worked in the field for more than 40 years, performing the first-ever in vitro synthesis of proteins by using ribosome components. Professor Spirin has received many honors for his scientific achievements, including the Hans Adolf Krebs Medal, and is the author of several books on ribosomes and protein synthesis. James Swartz is Professor of Chemical Engineering at Stanford University, California, USA. A graduate of the Massachusetts Institute of Technology, he has pioneered protein synthesis on an industrial scale while working for the companies Eli Lilly and Genentech. He is a leading expert on the biotechnological synthesis of mammalian proteins using bacterial whole cells and cell extracts.

Inhaltsangabe

Large scale batch reactions for cell-free protein synthesis (J. R. Swartz, A. Spirin) The constructive approach for cell-free translation (T. Ueda) Functional genomic analysis using sequential cell-free protein synthesis (J. R. Swartz) Cell-free technology for rapid production of patient-specific fusion protein vaccines (A. R. Goerke, J. Yang, G. Kanter, R. Levy and J. R. Swartz) Bacterial cell-free system for highly efficient protein synthesis (T. Kigawa, T. Matsuda, T. Yabuki, S. Yokoyama) The use of the Escherichia-coli cell-free protein synthesis for structural biology and structural proteomics (T. Kigawa, M. Inoue, M. Aoki, T. Matsuda, T. Yabuki, E. Seki, T. Harada, S. Watanabe, S. Yokoyama) The wheat germ cell-free protein synthesis system (T. Sawasaki, Y. Endo) Cell-free expression of integral membrane proteins for structural studies (C. Klammt, D. Schwarz, I. Lehner, S. Sobhanifar, F. Löhr, J. Zeelen, C. Glaubitz, V. Dötsch, F. Bernhard) Cell-free production of membrane protein in presence of detergents (J. Betton, M. Miot) Novel techniques using PCR and cell-free protein synthesis systems for combinatorial bioengineering (H. Nakano, T. Yamane) Gene Cloning and Expression in Molecular Colonies (A. B. Chetverin, T. R. Samatov, H. V. Chetverina)