Thomas Ming Swi Chang

Biomedical Applications of Immobilized Enzymes and Proteins (eBook, PDF)

Volume 1

• Format: PDF

Produktbeschreibung

I) ADSORPTION EEEEEEEE E E carrier 2) COVALENT LINKAGE a) Insoluble support b) Intermolecular linkage N'E~ ~~ c) Soluble support 0 \:)....m 3) tM TRIX (MOLECULAR) ENTRAPMENT ~~~~~;;..,J~-polymer matrix 4) ENCAPSULATION membrane FIGURE I. Classification of immobilized enzymes. Covalently linked, adsorbed, and matrix-entrapped enzymes represent stage II, research on the microenvironment. Microencapsulation represents stage III, research on the intracellular environment. Further subdivision of microencapsulated enzymes will be found in Chapter 12. 4 T. M. S. CHANG matrix entrapment. In this section, detailed discussions will center on clinical analysis, urine analysis, monitoring of environmental pollution, radioimmune assay, enzyme-linked immunosorbent assay, enzyme electrodes, and other approaches involving immobilized enzymes, antibodies, and antigens. In the final section, research workers describe and discuss the perspectives of immobilized enzymes and proteins. Here, they speculate on the future potential of possible approaches, even though these may not have been extensively studied or tested at the laboratory stage. The biomedical applications of enzymes and proteins, especially in the thera peutic area, is in a very early stage of development. Much remains to be explored and studied, and the area is wide open for investigators interested in original research in a new interdisciplinary area. References Chang, T. M. S., 1972, Artificial Cells, Charles C. Thomas, Publisher, Springfield, Ill. Dunlop, R. B. (ed.), 1974, Immobilized Biochemicals and Affinity ChrOTIULtography, Plenum Press, N ew York.

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Produktdetails

• Verlag: Springer US
• Seitenzahl: 448
• Erscheinungstermin: 21. November 2013
• Englisch
• ISBN-13: 9781468426106
• Artikelnr.: 44059336

Inhaltsangabe

1 Introduction.- I. Classification and Chemistries of Immobilized Enzymes.- 2 Covalent Linkage: I. Enzymes Immobilized by Covalent Linkage on Insolubilized Supports.- 3 Covalent Linkage: II. Intramolecular Linkages.- 4 Covalent Linkage: III. Immobilization of Enzymes by Intermolecular Cross-Linking.- 5 Immobilization of Enzymes by Adsorption.- 6 Gel-Entrapment of Enzymes.- 7 Encapsulation of Enzymes, Cell Contents, Cells, Vaccines, Antigens, Antiserum, Cofactors, Hormones, and Proteins.- II. Experimental Applications in Therapy.- 8 Rationale and Strategies for the Therapeutic Applications of Immobilized Enzymes.- 9 l-Asparaginase as a Model for Enzyme Therapy of Substrate-Dependent Tumors.- 10 A Biomedical View of Enzyme Replacement Strategies in Genetic Disease.- 11 Experimental Therapy Using Semipermeable Microcapsules Containing Enzymes and Other Biologically Active Material.- 12 Stabilized Urease Microencapsulated.- 13 Liquid-Membrane-Encapsulated Enzymes.- 14 Liposomes as Carriers of Enzymes and Proteins in Medicine.- 15 Enzyme-Loaded Erythrocytes.- 16 Enzyme Entrapment in Erythrocytes and Liposomes for the Treatment of Lysosomal Storage Diseases.- 17 Strategy for Enzyme Therapy: Immobilization in Hypoallergenic Gel Versus Entrapment in Red Blood Cell.- 18 Immobilized Enzymes for Therapeutic Applications and for Large-Scale Production of Biologically Active Compounds.- 19 Artificial Kidney, Artificial Liver, and Detoxfiers Based on Artificial Cells, Immobilized Proteins, and Immobilized Enzymes.- 20 Removal of Bilirubin from Blood by Affinity-Competition Chromatography over Albumin-Agarose Gel.- 21 Membrane-Immobilized Liver Microsome Drug Detoxfier.- 22 Some in Vivo and in Vitro Studies of Biologically Active Molecules on Organic Matrixes for Potential TherapeuticApplications.- 23 Therapeutic Perspectives of Enzyme Reactors.- 24 Possible Roles of Enzymes in Development of a Fuel Cell Power Source for the Cardiac Pacemaker.- 25 The Use of Enzymes for Oxygenator Membranes.