Beta-Arrestins (eBook, PDF)

Methods and Protocols
Redaktion: Scott, Mark G. H.; Laporte, Stéphane A.

• Format: PDF

Produktbeschreibung

This volume looks at various approaches to study the pleiotropic roles of b-arrestins (b-arrs) in the control of signal transduction, and the resulting cellular and in vivo consequences that arise. The chapters in this book cover diverse topics around b-arrs such as their established roles in GPCR regulation and trafficking; regulatory scaffolding functions of b-arrs in MAPK signaling, cAMP hydrolysis and cytoskeletal dynamics; proteomic analysis of the b-arr interactome; mathematical modelling of b-arr signaling networks; functional selectivity involving biased ligands; nucleocytoplasmic trafficking and primary cilia-associated functions of b-arrs; conformational plasticity of b-arrs; and the roles of b-arrs in allergic inflammation, Type 2 Diabetes, Parkinson's disease, and cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Beta-Arrestins: Methods and Protocols is a valuable resource for researchers interested in learning more about the function and regulation of b-arrestins.

---

Dieser Download kann aus rechtlichen Gründen nur mit Rechnungsadresse in A, B, BG, CY, CZ, D, DK, EW, E, FIN, F, GR, HR, H, IRL, I, LT, L, LR, M, NL, PL, P, R, S, SLO, SK ausgeliefert werden.

Produktdetails

• Verlag: Springer New York
• Seitenzahl: 409
• Erscheinungstermin: 27. März 2019
• Englisch
• ISBN-13: 9781493991587
• Artikelnr.: 64702071

Inhaltsangabe

A Brief History of the b-Arrestins.- b-Arrestins: Multitask Scaffolds Orchestrating the Where and When In Cell Signaling.- Methods to Monitor the Trafficking of beta-Arrestin/G Protein-Coupled Receptor Complexes using Enhanced Bystander BRET.- Methods to Investigate b-Arrestin-Mediated Regulation of GPCR Function in Human Airway Smooth Muscle.- Measuring Recruitment of b-Arrestin to G Protein-Coupled Heterodimers using Bioluminescence Resonance Energy Transfer.- Detection of beta-Arrestin-Mediated G Protein-Coupled Receptor Ubiquitination using BRET.- Using In Vitro Pull-Down and In Cell Overexpression Assays to Study Protein Interactions with Arrestin.- Methods to Investigate Arrestins in Complex with Phosphodiesterases.- Methods to Characterize Protein Interactions with beta-arrestin in cellulo.- Methods to Investigate the beta-Arrestin-Mediated control of ARF6 Activation to Regulate Trafficking and Actin Cytoskeleton Remodeling.- Methods to Investigate the Roles of b-Arrestin-Dependent RalGDS Activation in GPCR-Stimulated Membrane Blebbing.- Methods to Determine Interaction Interfaces between b-Arrestins and Their Protein Partners.- Workflow Description to Dynamically Model beta-Arrestin Signaling Networks.- Proteomic Analysis of the beta-Arrestin Interactome.- Quantitating Ligand Bias using the Competitive Model of Ligand Activity.- Methods to Investigate the Nucleocytoplasmic Shuttling Properties of b-Arrestins.- Monitoring beta-Arrestin2 Targeting to the Centrosome, Basal Body and Primary Cilium by Fluorescence Microscopy.- A Mass Spectrometry-Based Structural Assay for Activation-Dependent Conformational Changes in beta-Arrestins.- Probing Arrestin Function using Intramolecular FlAsH-BRET Biosensors.- Methods to Study the Roles of b-Arrestins in Meningococcal Signaling.- Methods to Investigate the Roles for beta-Arrestin2 in Allergic Inflammatory Airway Disease.- Methods to Study Roles of beta-Arrestins in the Regulation of Pancreatic beta-Cell Function.- Methods to Investigate beta-Arrestin Function in Metabolic Regulation.- Methods to Investigate the Role of b-Arrestin Signaling in Parkinson's Ddisease.- Methods to Investigate beta-Arrestin-1/beta-Catenin Signaling in Ovarian Cancer Cells.